Journal Article

LncRNA H19 induced by helicobacter pylori infection promotes gastric cancer cell growth via enhancing NF-κB-induced inflammation

Yan, Jin, Zhang, Yifeng, Zhang, Ximei, Shen, Xiaoran, Li, Chao, Dong, Yu, Wang, Xiaoyong

2019

Overview

Background The aim of this study was to investigate the role of long non-coding RNA (lncRNA) H19 in gastric cancer (GC) with Helicobacter pylori ( H. pylori ). Methods H19 expression in peripheral blood from H. pylori +/− GC patients and healthy donors (control) as well as in GC tissues and cells were detected by qRT-PCR. Cell proliferation was evaluated by CCK-8 assay. Cell migration and invasion were evaluated by Transwell assay. The levels of pro-inflammatory cytokines were determined by ELISA. The protein levels of IκBα, p-IκBα and p65 were determined by western blotting. Results H19 expression was upregulated in H. pylori -infected GC tissues and cells. Furthermore, H. pylori promoted GC cell viability, migration, invasion and inflammatory response. Moreover, H19 overexpression promoted the proliferation, migration and invasion of H. pylori -infected GC cells via enhancing NF-κB-induced inflammation. Conclusions LncRNA H19 promotes H. pylori -induced GC cell growth via enhancing NF-κB-induced inflammation.

• Publisher: BioMed Central,Springer Nature B.V,BMC
• Subject: Allergology / Antibiotics / Biomedical and Life Sciences / Biomedicine / Cell adhesion & migration / Cell growth / Cell migration / Cell proliferation / Cell viability / Cholecystokinin / Cytokines / Cytokines and Growth Factors / Enzyme-linked immunosorbent assay / Gastric cancer / Gastroenterology / H. pylori / H19 / Helicobacter pylori / Immunology / Infections / Inflammation / Kinases / Medical prognosis / Medical research / Metastasis / NF-κB protein / NF-κB signaling pathway / Non-coding RNA / Peripheral blood / Pharmacology/Toxicology / Proteins / Rheumatology / Western blotting