Journal Article

FOXP3 promotes tumor growth and metastasis by activating Wnt/β-catenin signaling pathway and EMT in non-small cell lung cancer

Underwood, Malcolm J., Dong, Yujuan, Wang, Shanshan, Wan, Innes Y. P., Long, Xiang, Yang, Shucai, Yang, Sheng-li, Mok, Tony, Li, Ming-Yue, Zou, Chang, Ng, Calvin S. H., Liu, Yi, Chen, George G., Du, Jing, Liu, Li-Zhong

2017

Overview

Background The role of cancer cell FOXP3 in tumorigenesis is conflicting. We aimed to study FOXP3 expression and regulation, function and clinical implication in human non-small cell lung cancer (NSCLC). Methods One hundred and six patients with histologically-confirmed NSCLC who underwent surgery were recruited for the study. Tumor samples and NSCLC cell lines were used to examine FOXP3 and its related molecules. Various cell functions related to tumorigenesis were performed. In vivo mouse tumor xenograft was used to confirm the in vitro results. Results NSCLC patients with the high level of FOXP3 had a significant decrease in overall survival and recurrence-free survival. FOXP3 overexpression significantly induced cell proliferation, migration, and invasion, whereas its inhibition impaired its oncogenic function. In vivo studies confirmed that FOXP3 promoted tumor growth and metastasis. The ectopic expression of FOXP3 induced epithelial–mesenchymal transition (EMT) with downregulation of E-cadherin and upregulation of N-cadherin, vimentin, snail, slug, and MMP9. The oncogenic effects by FOXP3 could be attributed to FOX3-mediated activation of Wnt/β-catenin signaling, as FOXP3 increased luciferase activity of Topflash reporter and upregulated Wnt signaling target genes including c-Myc and Cyclin D1 in NSCLC cells. Co-immunoprecipitation results further indicated that FOXP3 could physically interacted with β-catenin and TCF4 to enhance the functions of β-catenin and TCF4, inducing transcription of Wnt target genes to promote cell proliferation, invasion and EMT induction. Conclusions FOXP3 can act as a co-activator to facilitate the Wnt-b-catenin signaling pathway, inducing EMT and tumor growth and metastasis in NSCLC.

• Publisher: BioMed Central,Springer Nature B.V,BMC
• Subject: Animals / Apoptosis / Biomedical and Life Sciences / Biomedicine / Breast cancer / c-Myc protein / Cancer Research / Carcinogenesis - pathology / Carcinoma, Non-Small-Cell Lung - metabolism / Carcinoma, Non-Small-Cell Lung - pathology / Cell Line, Tumor / Cell lines / Cell Proliferation / Cyclin D1 / E-cadherin / Ectopic expression / EMT / Epithelial-Mesenchymal Transition / Forkhead Transcription Factors - metabolism / FOXP3 / Foxp3 protein / Gelatinase B / Gene expression / HEK293 Cells / Humans / Immunoglobulins / Immunoprecipitation / Lung cancer / Lung Neoplasms - metabolism / Lung Neoplasms - pathology / Lymphatic system / Medical prognosis / Mesenchyme / Metastases / Metastasis / Mice, Nude / Myc protein / N-Cadherin / Neoplasm Metastasis / Non-small cell lung carcinoma / NSCLC / Oncology / Prognosis / Prostate / Rodents / Signal transduction / Studies / Surgery / Survival / TCF4 / Transcription / Tumor cell lines / Tumorigenesis / Vimentin / Wnt / Wnt protein / Wnt Signaling Pathway / Xenografts / β-Catenin