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Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm
by
Shokrollahi, Neda
, Ho, Chai-Ling
, Wahab, Mohd As’wad Bin Abul
, Wong, Mui-Yun
, Zainudin, Nur Ain Izzati Mohd
in
631/208/8
/ 631/326/193
/ 631/449/2169
/ 631/449/2169/2673
/ DNA, Plant - genetics
/ Ganoderma - genetics
/ Ganoderma - metabolism
/ Ganoderma boninense
/ Gene expression
/ Genes, Plant - genetics
/ Humanities and Social Sciences
/ Inoculation
/ Metabolites
/ multidisciplinary
/ NRPS gene
/ Palm Oil - metabolism
/ Pathogenesis
/ Peptide Synthases - genetics
/ Peptide Synthases - metabolism
/ Peptides
/ Phylogeny
/ Plant Proteins - genetics
/ Plant Proteins - metabolism
/ Plant Roots - genetics
/ Plant Roots - metabolism
/ Science
/ Science (multidisciplinary)
/ Secondary metabolites
/ Seedlings
/ Seedlings - genetics
/ Seedlings - metabolism
/ Stem rot
/ Vegetable oils
/ White rot
2021
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Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm
by
Shokrollahi, Neda
, Ho, Chai-Ling
, Wahab, Mohd As’wad Bin Abul
, Wong, Mui-Yun
, Zainudin, Nur Ain Izzati Mohd
in
631/208/8
/ 631/326/193
/ 631/449/2169
/ 631/449/2169/2673
/ DNA, Plant - genetics
/ Ganoderma - genetics
/ Ganoderma - metabolism
/ Ganoderma boninense
/ Gene expression
/ Genes, Plant - genetics
/ Humanities and Social Sciences
/ Inoculation
/ Metabolites
/ multidisciplinary
/ NRPS gene
/ Palm Oil - metabolism
/ Pathogenesis
/ Peptide Synthases - genetics
/ Peptide Synthases - metabolism
/ Peptides
/ Phylogeny
/ Plant Proteins - genetics
/ Plant Proteins - metabolism
/ Plant Roots - genetics
/ Plant Roots - metabolism
/ Science
/ Science (multidisciplinary)
/ Secondary metabolites
/ Seedlings
/ Seedlings - genetics
/ Seedlings - metabolism
/ Stem rot
/ Vegetable oils
/ White rot
2021
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Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm
by
Shokrollahi, Neda
, Ho, Chai-Ling
, Wahab, Mohd As’wad Bin Abul
, Wong, Mui-Yun
, Zainudin, Nur Ain Izzati Mohd
in
631/208/8
/ 631/326/193
/ 631/449/2169
/ 631/449/2169/2673
/ DNA, Plant - genetics
/ Ganoderma - genetics
/ Ganoderma - metabolism
/ Ganoderma boninense
/ Gene expression
/ Genes, Plant - genetics
/ Humanities and Social Sciences
/ Inoculation
/ Metabolites
/ multidisciplinary
/ NRPS gene
/ Palm Oil - metabolism
/ Pathogenesis
/ Peptide Synthases - genetics
/ Peptide Synthases - metabolism
/ Peptides
/ Phylogeny
/ Plant Proteins - genetics
/ Plant Proteins - metabolism
/ Plant Roots - genetics
/ Plant Roots - metabolism
/ Science
/ Science (multidisciplinary)
/ Secondary metabolites
/ Seedlings
/ Seedlings - genetics
/ Seedlings - metabolism
/ Stem rot
/ Vegetable oils
/ White rot
2021
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Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm
Journal Article
Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm
2021
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Overview
Basal stem rot (BSR) of oil palm is a disastrous disease caused by a white-rot fungus
Ganoderma boninense
Pat. Non-ribosomal peptides (NRPs) synthesized by non-ribosomal peptide synthetases (NRPSs) are a group of secondary metabolites that act as fungal virulent factors during pathogenesis in the host. In this study, we aimed to isolate NRPS gene of
G. boninense
strain UPMGB001 and investigate the role of this gene during
G. boninense
-oil palm interaction. The isolated NRPS DNA fragment of 8322 bp was used to predict the putative peptide sequence of different domains and showed similarity with
G. sinense
(85%) at conserved motifs of three main NRPS domains. Phylogenetic analysis of NRPS peptide sequences demonstrated that NRPS of
G. boninense
belongs to the type VI siderophore family. The roots of 6-month-old oil palm seedlings were artificially inoculated for studying NRPS gene expression and disease severity in the greenhouse. The correlation between high disease severity (50%) and high expression (67-fold) of
G. boninense
NRPS gene at 4 months after inoculation and above indicated that this gene played a significant role in the advancement of BSR disease. Overall, these findings increase our knowledge on the gene structure of NRPS in
G. boninense
and its involvement in BSR pathogenesis as an effector gene.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
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