Journal Article

Interleukin-22 regulates neutrophil recruitment in ulcerative colitis and is associated with resistance to ustekinumab therapy

Ding, Yuchun, Yang, Feifei, Pavlidis, Polychronis, Niazi, Umar, Powell, Nick, Lamb, Christopher, Lo, Jonathan W., Cozzetto, Domenico, Tsakmaki, Anastasia, Bewick, Gavin, Pantazi, Eirini, Li, Katherine, Macdonald, Thomas T., Long, Anna K., Lord, Graham M., Madgwick, Matthew, Alberts, Elena, Friedman, Joshua, Sa, Ana Caroline Costa, Saqi, Mansoor, Treveil, Agatha, Digby- Bell, Jonathan, Carey, Christopher D., Gul, Leila, Korcsmaros, Tamas

2022

Overview

The function of interleukin-22 (IL-22) in intestinal barrier homeostasis remains controversial. Here, we map the transcriptional landscape regulated by IL-22 in human colonic epithelial organoids and evaluate the biological, functional and clinical significance of the IL-22 mediated pathways in ulcerative colitis (UC). We show that IL-22 regulated pro-inflammatory pathways are involved in microbial recognition, cancer and immune cell chemotaxis; most prominently those involving CXCR2 + neutrophils. IL-22-mediated transcriptional regulation of CXC-family neutrophil-active chemokine expression is highly conserved across species, is dependent on STAT3 signaling, and is functionally and pathologically important in the recruitment of CXCR2 + neutrophils into colonic tissue. In UC patients, the magnitude of enrichment of the IL-22 regulated transcripts in colonic biopsies correlates with colonic neutrophil infiltration and is enriched in non-responders to ustekinumab therapy. Our data provide further insights into the biology of IL-22 in human disease and highlight its function in the regulation of pathogenic immune pathways, including neutrophil chemotaxis. The transcriptional networks regulated by IL-22 are functionally and clinically important in UC, impacting patient trajectories and responsiveness to biological intervention. Mechanisms of non-response to ustekinumab, a biologic targeting IL-23, are currently unclear. Here, the authors show that the transcriptional program regulated by IL-22, an IL-23 responsive cytokine, is enriched in patients with ulcerative colitis unresponsive to ustekinumab and associated with higher colon neutrophil recruitment and activation of upstream IL-22 regulators.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 13/100 / 13/21 / 13/31 / 38/61 / 38/91 / 631/250/127/1213 / 631/250/2502/2055 / 631/250/2504/223/1699 / 631/250/347 / 64 / 64/60 / 692/4020/1503/257 / Biopsy / Cell activation / Chemokines / Chemokines, CXC - metabolism / Chemotaxis / Colitis, Ulcerative - drug therapy / Colitis, Ulcerative - genetics / Colon / CXCR2 protein / Cytokines / Enrichment / Gene regulation / Homeostasis / Humanities and Social Sciences / Humans / Immune system / Inflammatory bowel disease / Inflammatory bowel diseases / Interleukin 22 / Interleukin 23 / Interleukin-8 - metabolism / Interleukins / Leukocytes (neutrophilic) / Microorganisms / Monoclonal antibodies / multidisciplinary / Neutrophil Infiltration / Neutrophils / Neutrophils - metabolism / Organoids / Patients / Receptors, Interleukin-8B - metabolism / Recruitment / Science / Science (multidisciplinary) / Stat3 protein / Transcription / Ulcerative colitis / Ustekinumab - pharmacology / Ustekinumab - therapeutic use