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Extensive co-binding and rapid redistribution of NANOG and GATA6 during emergence of divergent lineages
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Extensive co-binding and rapid redistribution of NANOG and GATA6 during emergence of divergent lineages
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Journal Article
Extensive co-binding and rapid redistribution of NANOG and GATA6 during emergence of divergent lineages
2022
Overview
Fate-determining transcription factors (TFs) can promote lineage-restricted transcriptional programs from common progenitor states. The inner cell mass (ICM) of mouse blastocysts co-expresses the TFs NANOG and GATA6, which drive the bifurcation of the ICM into either the epiblast (Epi) or the primitive endoderm (PrE), respectively. Here, we induce GATA6 in embryonic stem cells–that also express NANOG–to characterize how a state of co-expression of opposing TFs resolves into divergent lineages. Surprisingly, we find that GATA6 and NANOG co-bind at the vast majority of Epi and PrE enhancers, a phenomenon we also observe in blastocysts. The co-bound state is followed by eviction and repression of Epi TFs, and quick remodeling of chromatin and enhancer-promoter contacts thus establishing the PrE lineage while repressing the Epi fate. We propose that co-binding of GATA6 and NANOG at shared enhancers maintains ICM plasticity and promotes the rapid establishment of Epi- and PrE-specific transcriptional programs.
The authors show that the transcription factors NANOG and GATA6 co-bind the same enhancers in common progenitors before divergent epiblast and primitive endoderm lineages emerge. This may help maintain plasticity at early stages and facilitate bifurcation into distinct lineages
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 45/77
/ Animals
/ Binding
/ Cell Differentiation - genetics
/ Endoderm
/ GATA6 Transcription Factor - genetics
/ GATA6 Transcription Factor - metabolism
/ Gene Expression Regulation, Developmental
/ Humanities and Social Sciences
/ Mice
/ Nanog Homeobox Protein - genetics
/ Nanog Homeobox Protein - metabolism
/ Science
