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Mast4 knockout shows the regulation of spermatogonial stem cell self-renewal via the FGF2/ERM pathway
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Mast4 knockout shows the regulation of spermatogonial stem cell self-renewal via the FGF2/ERM pathway
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Journal Article
Mast4 knockout shows the regulation of spermatogonial stem cell self-renewal via the FGF2/ERM pathway
2021
Overview
Spermatogenesis is an important cellular differentiation process that produces the male gametes and remains active throughout the individual’s lifespan. Sertoli cell-only syndrome (SCO) refers to the dysfunction of the male reproductive system, including infertility. Accurate self-renewal of spermatogonial stem cells (SSCs) is essential to prevent SCO syndrome. This study investigated the role of microtubule-associated serine/threonine kinase family member 4 (MAST4) in spermatogenesis in mice. MAST4 was localized in Sertoli cells before puberty, providing a somatic niche for spermatogenesis in mice and MAST4 expression shifted to Leydig cells and spermatids throughout puberty.
Mast4
knockout (KO) testes were reduced in size compared to wild-type testes, and germ cell depletion associated with an increase in apoptosis and subsequent loss of tubular structure were similar to the SCO phenotype. In addition, MAST4 phosphorylated the Ets-related molecule (ERM), specifically the serine 367 residue. The phosphorylation of ERM ultimately controls the transcription of ERM target genes related to SSC self-renewal. The expression of spermatogenesis-associated proteins was significantly decreased whereas Sertoli cell markers were increased in
Mast4
KO testes, which was well-founded by RNA-sequencing analysis. Therefore, MAST4 is associated with the fibroblast growth factor 2 (FGF2)/ERM pathway and this association helps us explore the capacity of SSCs to maintain a vertebrate stem cell niche.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 38/91
/ 42/109
/ 64/110
/ 64/60
/ 82/51
/ Animals
/ Biomedical and Life Sciences
/ Fibroblast Growth Factor 2 - metabolism
/ Gametes
/ Humans
/ Male
/ Mice
/ Microtubule-Associated Proteins - metabolism
/ Protein Serine-Threonine Kinases - metabolism
/ Protein-serine/threonine kinase
/ Puberty
/ Testes
