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A multiplex serologic platform for diagnosis of tick-borne diseases
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A multiplex serologic platform for diagnosis of tick-borne diseases
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A multiplex serologic platform for diagnosis of tick-borne diseases
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Journal Article
A multiplex serologic platform for diagnosis of tick-borne diseases
2018
Overview
Tick-borne diseases are the most common vector-borne diseases in the United States, with serology being the primary method of diagnosis. We developed the first multiplex, array-based assay for serodiagnosis of tick-borne diseases called the TBD-Serochip. The TBD-Serochip was designed to discriminate antibody responses to 8 major tick-borne pathogens present in the United States, including
Anaplasma phagocytophilum, Babesia microti
,
Borrelia burgdorferi
,
Borrelia miyamotoi
,
Ehrlichia chaffeensis
,
Rickettsia rickettsii
, Heartland virus and Powassan virus. Each assay contains approximately 170,000 12-mer linear peptides that tile along the protein sequence of the major antigens from each agent with 11 amino acid overlap. This permits accurate identification of a wide range of specific immunodominant IgG and IgM epitopes that can then be used to enhance diagnostic accuracy and integrate differential diagnosis into a single assay. To test the performance of the TBD-Serochip, we examined sera from patients with confirmed Lyme disease, babesiosis, anaplasmosis, and Powassan virus disease. We identified a wide range of specific discriminatory epitopes that facilitated accurate diagnosis of each disease. We also identified previously undiagnosed infections. Our results indicate that the TBD-Serochip is a promising tool for a differential diagnosis not available with currently employed serologic assays for TBDs.
Publisher
Nature Publishing Group UK,Nature Publishing Group
