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LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution
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LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution
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Journal Article
LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution
2020
Overview
Many bacteria employ a type III secretion system (T3SS) injectisome to translocate proteins into eukaryotic host cells. Although the T3SS can efficiently export heterologous cargo proteins, a lack of target cell specificity currently limits its application in biotechnology and healthcare. In this study, we exploit the dynamic nature of the T3SS to govern its activity. Using optogenetic interaction switches to control the availability of the dynamic cytosolic T3SS component SctQ, T3SS-dependent effector secretion can be regulated by light. The resulting system, LITESEC-T3SS (Light-induced translocation of effectors through sequestration of endogenous components of the T3SS), allows rapid, specific, and reversible activation or deactivation of the T3SS upon illumination. We demonstrate the light-regulated translocation of heterologous reporter proteins, and induction of apoptosis in cultured eukaryotic cells. LITESEC-T3SS constitutes a new method to control protein secretion and translocation into eukaryotic host cells with unparalleled spatial and temporal resolution.
The type III secretion system (T3SS) of bacteria can be used to inject cargo into eukaryotic cells but its lack of target specificity is a disadvantage. Here the authors place the T3SS under the regulation of light by engineering optogenetic switches into the dynamic cytosolic T3SS component SctQ.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 14/35
/ 14/63
/ Bacteria
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Cargo
/ Eukaryotic Cells - metabolism
/ Eukaryotic Cells - microbiology
/ Gene Expression Regulation, Bacterial
/ Gram-Negative Bacteria - genetics
/ Gram-Negative Bacteria - metabolism
/ Gram-Negative Bacteria - physiology
/ Humanities and Social Sciences
/ Humans
/ Light
/ Protein Transport - radiation effects
/ Proteins
/ Science
/ Switches
/ Type III Secretion Systems - genetics
/ Type III Secretion Systems - metabolism
/ Yersinia enterocolitica - genetics
