Journal Article

Structure and assembly mechanisms of the microbial community on an artificial reef surface, Fangchenggang, China

Zhang, Ankai, Guo, Yu, Zou, Jian, Qin, Chuanxin, Ma, Zhenhua, Yu, Gang, Shao, Guangyu

2025

Overview

The construction of artificial reefs (ARs) is an effective way to restore habitats and increase and breed fishery resources in marine ranches. However, studies on the impacts of ARs on the structure, function, and assembly patterns of the bacterial community (BC), which is important in biogeochemical cycles, are lacking. The compositions, diversities, assembly patterns, predicted functions, and key environmental factors of the attached and free-living microbial communities in five-year ARs (O-ARs) and one-year ARs (N-ARs) in Fangchenggang, China, were analyzed via 16S rRNA gene sequencing. Proteobacteria was the dominant taxon in all the samples, with an average relative abundance of 44.48%, followed by Bacteroidetes (17.42%) and Cyanobacteria (15.19%). The composition of bacterial phyla was similar between O-ARs and N-ARs, but the relative abundance of Cyanobacteria was greater in the water column (38.56%) than on the AR surface (mean of 7.40%). The results revealed that the Shannon‒Wiener diversity indices were 5.64 and 5.45 for O-ARs and N-ARs, respectively. Principal coordinate analysis (PCoA) revealed different distributions of O-ARs and N-ARs in the microbial community. Additionally, network analysis revealed that the bacterial community was more complex and stable in O-ARs than in N-ARs, indicating that the 5-year AR presented a more diverse and stable microbial community overall. The KEGG database was used to predict that nitrogen metabolism, carbon metabolism, and membrane transport were the dominant microbial functions, accounting for 29.93% of the total functional abundances. The results of the neutral community model revealed that stochastic processes (67.2%) dominated the assembly of BCs. Interestingly, deterministic processes may be increasingly important in community aggregation over time. Moreover, a null model revealed that dispersal limitation was the most important process among the stochastic processes, accounting for 57.14% of the total. In addition, redundancy analysis (RDA) revealed that hydrological factors obviously impacted the structure and function of the microbial community. Our results showed that the construction of ARs slightly promotes local diversities in the structure and function of the microbial community, indicating it requires a longer time to enhance the diversity of the microbial community on artificial reefs. Key points • Artificial reefs facilitate the diversity and functions of the microbial community • Stochastic processes dominate the assembly of the microbial community in artificial reefs • Nitrogen and carbon metabolism dominate microbial functions in artificial reefs

• Publisher: Springer Berlin Heidelberg,Springer Nature B.V
• Subject: Abundance / Artificial reefs / Assembly / Bacteria - classification / Bacteria - genetics / Bacteria - isolation & purification / bacterial communities / Bacteroidetes / Bacteroidetes - classification / Bacteroidetes - genetics / Bacteroidetes - isolation & purification / Biodiversity / Biogeochemical cycles / Biomedical and Life Sciences / Biotechnology / Carbon / carbon metabolism / China / Composition / Coral Reefs / Cyanobacteria / Cyanobacteria - classification / Cyanobacteria - genetics / Cyanobacteria - isolation & purification / Diversity indices / DNA, Bacterial - chemistry / DNA, Bacterial - genetics / Environmental Biotechnology / Environmental factors / Fisheries / Fishery resources / Functionals / Gene sequencing / genes / hydrology / Life Sciences / Marine resources / Metabolism / Microbial activity / Microbial Genetics and Genomics / Microbiology / Microbiomes / Microbiota / Microorganisms / multidimensional scaling / Network analysis / Nitrogen / Nitrogen metabolism / Phylogeny / Proteobacteria / Proteobacteria - classification / Proteobacteria - genetics / Proteobacteria - isolation & purification / Recovery of function / Redundancy / Relative abundance / RNA, Ribosomal, 16S - genetics / rRNA 16S / Seawater - microbiology / Sequence Analysis, DNA / Stochastic models / Stochastic processes / Structure-function relationships / Water circulation / Water column