Journal Article

Silencing the FABP3 gene in insulin-secreting cells reduces fatty acid uptake and protects against lipotoxicity

Hyder, Ayman, Schrezenmeir, Jürgen, Sheta, Basma, Eissa, Manar

2024

Overview

Background Long-term exposure of pancreatic islets to fatty acids (FAs), common in obesity, metabolic syndrome, and type 2 diabetes, leads to a compensatory hyperactivity followed by inflammation, apoptosis, dysfunctional beta cells, and results in insulin dependence of the patient. Restriction of fatty uptake by islet beta cells may protect them from lipotoxicity. Purpose Pancreatic islet beta cells express the fatty acid binding protein 3 (FABP3) to bind FAs and to orchestrate lipid signals. Based on this, we investigated whether downregulation of FABP3, by Fabp3 silencing, might slow lipid metabolism and protect against lipotoxicity in insulin-secreting cells. Results Neither Fabp3 silencing, nor overexpression affected the glucose-stimulated insulin secretion in absence of FAs. Fabp3 silencing decreased FA-uptake, lipid droplets formation, and the expression of the lipid accumulation-regulating gene Dgat1 in Ins1E cells. It reduced FA-induced inflammation by deactivation of NF-κB, which was associated with upregulation of IκBα and deactivation of the NF-κB p65 nuclear translocation, and the downregulation of the cytokines ILl-6, IL-1β, and TNFα. Ins1E cells were protected from the FA-induced apoptosis as assessed by different parameters including DNA degradation and cleaved caspase-3 immunoblotting. Furthermore, FABP3 silencing improved the viability, Pdx1 gene expression, and the insulin-secreting function in cells long-term cultured with palmitic acid. All results were confirmed by the opposite action rendered by FABP3 overexpression. Conclusion The present data reveals that pancreatic beta cells can be protected from lipotoxicity by inhibition of FA-uptake, intracellular utilization and accumulation. FABP3 inhibition, hence, may be a useful pharmaceutical approach in obesity, metabolic syndrome, and type 2 diabetes.

• Publisher: Springer Milan,Springer Nature B.V
• Subject: Animals / Apoptosis / Beta cells / Caspase-3 / Cloning / Diabetes / Diabetes mellitus (non-insulin dependent) / DNA methylation / Down-regulation / Fatty Acid Binding Protein 3 - genetics / Fatty Acid Binding Protein 3 - metabolism / Fatty acid-binding protein / Fatty Acid-Binding Proteins - genetics / Fatty Acid-Binding Proteins - metabolism / Fatty acids / Fatty Acids - metabolism / Gene expression / Gene Silencing / Humans / Hyperactivity / Immunoblotting / Inflammation / Insulin - metabolism / Insulin resistance / Insulin secretion / Insulin Secretion - genetics / Insulin-Secreting Cells - metabolism / Internal Medicine / Lipid metabolism / Lipid Metabolism - genetics / Lipids / Medicine / Medicine & Public Health / Metabolic Diseases / Metabolic syndrome / Mice / NF-κB protein / Nuclear transport / Obesity / Original / Original Article / Palmitic acid / Pancreas / Proteins / Rats / Reagents / Thermal cycling