Asset Details
Do you wish to reserve the book?
SMC translocation is unaffected by an excess of nucleoid associated proteins in vivo
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
SMC translocation is unaffected by an excess of nucleoid associated proteins in vivo
Do you wish to request the book?
SMC translocation is unaffected by an excess of nucleoid associated proteins in vivo
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
SMC translocation is unaffected by an excess of nucleoid associated proteins in vivo
2025
Overview
Genome organization is important for DNA replication, gene expression, and chromosome segregation. In bacteria, two large families of proteins, nucleoid-associated proteins (NAPs) and SMC complexes, play important roles in organizing the genome. NAPs are highly abundant DNA-binding proteins that can bend, wrap, bridge, and compact DNA, while SMC complexes load onto the chromosome, translocate on the DNA, and extrude DNA loops. Although SMC complexes are capable of traversing the entire chromosome bound by various NAPs in vivo, it is unclear whether SMC translocation is influenced by NAPs. In this study, using
Bacillus subtilis
as a model system, we expressed a collection of representative bacterial and archaeal DNA-binding proteins that introduce distinct DNA structures and potentially pose different challenges for SMC movement. By fluorescence microscopy and chromatin immunoprecipitation, we observed that these proteins bound to the genome in characteristic manners. Using genome-wide chromosome conformation capture (Hi-C) assays, we found that the SMC complex traversed these DNA-binding proteins without slowing down. Our findings revealed that the DNA-loop-extruding activity of the SMC complex is unaffected by exogenously expressed DNA-binding proteins, which highlights the robustness of SMC motors on the busy chromatin.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 631/326
/ 631/337
/ 631/80
/ Bacillus subtilis - genetics
/ Bacillus subtilis - metabolism
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Cell Cycle Proteins - metabolism
/ Chromosomes, Bacterial - genetics
/ Chromosomes, Bacterial - metabolism
/ DNA
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - metabolism
/ Genomes
/ H-NS
/ HBsu
/ Humanities and Social Sciences
/ NAP
/ Nucleoid-associated proteins
/ Proteins
/ Science
/ SMC
