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Cytoneme-mediated transport of active Wnt5b–Ror2 complexes in zebrafish
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Cytoneme-mediated transport of active Wnt5b–Ror2 complexes in zebrafish
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Cytoneme-mediated transport of active Wnt5b–Ror2 complexes in zebrafish
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Journal Article
Cytoneme-mediated transport of active Wnt5b–Ror2 complexes in zebrafish
2024
Overview
Chemical signalling is the primary means by which cells communicate in the embryo. The underlying principle refers to a group of ligand-producing cells and a group of cells that respond to this signal because they express the appropriate receptors
1
,
2
. In the zebrafish embryo, Wnt5b binds to the receptor Ror2 to trigger the Wnt–planar cell polarity (PCP) signalling pathway to regulate tissue polarity and cell migration
3
,
4
. However, it remains unclear how this lipophilic ligand is transported from the source cells through the aqueous extracellular space to the target tissue. In this study, we provide evidence that Wnt5b, together with Ror2, is loaded on long protrusions called cytonemes. Our data further suggest that the active Wnt5b–Ror2 complexes form in the producing cell and are handed over from these cytonemes to the receiving cell. Then, the receiving cell has the capacity to initiate Wnt–PCP signalling, irrespective of its functional Ror2 receptor status. On the tissue level, we further show that cytoneme-dependent spreading of active Wnt5b–Ror2 affects convergence and extension in the zebrafish gastrula. We suggest that cytoneme-mediated transfer of ligand–receptor complexes is a vital mechanism for paracrine signalling. This may prompt a reevaluation of the conventional concept of characterizing responsive and non-responsive tissues solely on the basis of the expression of receptors.
In zebrafish embryos, active complexes of Wnt5b and its membrane-bound receptor Ror2 are transported between cells via cellular protrusions called cytonemes to initiate paracrine Wnt5b signalling in cells that do not endogenously express the receptor.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
