Journal Article

UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean

Zhang, Ke, Xiang, Fengning, Kong, Fanjiang, Zhang, Wenxiao, Zheng, Wenyu, Li, Shuo, Liu, Baohui, Feng, Xianzhong, Huang, Shiyu, Zheng, Xiaojian, Sun, Jiaqi, Wang, Lili, Zhou, Qian, Li, Jie, Zhao, Shizhen, Lu, Qing

2023

Overview

Plant height is a key agronomic trait that affects yield and is controlled by both phytohormone gibberellin (GA) and ultraviolet-B (UV-B) irradiation. However, whether and how plant height is modulated by UV-B-mediated changes in GA metabolism are not well understood. It has not been reported that the E3 ubiquitin ligase Anaphase Promoting Complex/Cyclosome (APC/C) is involved in the regulation of plant growth in response to environmental factors. We perform a forward genetic screen in soybean and find that a mutation in Glycine max Increased Leaf Petiole Angle1 ( GmILPA1 ), encoding a subunit of the APC/C, lead to dwarfism under UV-B irradiation. UV-B promotes the accumulation of GmILPA1, which ubiquitinate the GA catabolic enzyme GA2 OXIDASE-like (GmGA2ox-like), resulting in its degradation in a UV-B-dependent manner. Another E3 ligase, GmUBL1, also ubiquitinate GmGA2ox-like and enhance the GmILPA1-mediated degradation of GmGA2ox-like, which suggest that GmILPA1-GmGA2ox-like module counteract the UV-B-mediated reduction of bioactive GAs. We also determine that GmILPA1 is a target of selection during soybean domestication and breeding. The deletion (Indel-665) in the promoter might facilitate the adaptation of soybean to high UV-B irradiation. This study indicates that an evolutionary GmILPA1 variant has the capability to develop ideal plant architecture with soybean cultivars. Gibberellins (GA) negatively regulate UVB-mediated suppression of plant height in plants. Here, the authors show that GmILPA1-mediated degradation of GmGA2ox-like protein counteract the UV-B-mediated reduction of bioactive GAs contributing to the linkage between the affection of UV-B and GA on plant height of soybean.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 13/1 / 38/77 / 631/449/1741/1521 / 631/449/711 / 631/45/776 / 82/111 / Anaphase / Anaphase-promoting complex / Anaphase-Promoting Complex-Cyclosome / Biological activity / Catabolism / Cultivars / Degradation / Domestication / Dwarfism / Environmental factors / Gene deletion / Genetic screening / Gibberellins / Glycine max / Glycine max - genetics / Glycine max - metabolism / Humanities and Social Sciences / Irradiation / multidisciplinary / Plant Breeding / Plant growth / Plant hormones / Plant Leaves - metabolism / Plants - metabolism / Radiation / Reduction / Science / Science (multidisciplinary) / Soybeans / Ubiquitin-protein ligase / Ubiquitin-Protein Ligases - genetics / Ubiquitin-Protein Ligases - metabolism / Ultraviolet radiation