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Molecular mechanism of phospholipid transport at the bacterial outer membrane interface
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Molecular mechanism of phospholipid transport at the bacterial outer membrane interface
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Journal Article
Molecular mechanism of phospholipid transport at the bacterial outer membrane interface
2023
Overview
The outer membrane (OM) of Gram-negative bacteria is an asymmetric lipid bilayer with outer leaflet lipopolysaccharides and inner leaflet phospholipids (PLs). This unique lipid asymmetry renders the OM impermeable to external insults, including antibiotics and bile salts. To maintain this barrier, the OmpC-Mla system removes mislocalized PLs from the OM outer leaflet, and transports them to the inner membrane (IM); in the first step, the OmpC-MlaA complex transfers PLs to the periplasmic chaperone MlaC, but mechanistic details are lacking. Here, we biochemically and structurally characterize the MlaA-MlaC transient complex. We map the interaction surfaces between MlaA and MlaC in
Escherichia coli
, and show that electrostatic interactions are important for MlaC recruitment to the OM. We further demonstrate that interactions with MlaC modulate conformational states in MlaA. Finally, we solve a 2.9-Å cryo-EM structure of a disulfide-trapped OmpC-MlaA-MlaC complex in nanodiscs, reinforcing the mechanism of MlaC recruitment, and highlighting membrane thinning as a plausible strategy for directing lipids for transport. Our work offers critical insights into retrograde PL transport by the OmpC-Mla system in maintaining OM lipid asymmetry.
Maintenance of lipid asymmetry in the bacterial outer membrane (OM) is mediated by the OmpC-Mla system, but mechanistic details remain to be elucidated. Here, the authors show that electrostatic interactions, conformational dynamics, and membrane thinning may facilitate spontaneous retrograde phospholipid transfer at the OM.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 38/70
/ 82/16
/ 82/80
/ 82/83
/ Bacteria
/ Bacterial Outer Membrane - metabolism
/ Bacterial Outer Membrane Proteins - metabolism
/ E coli
/ Escherichia coli - metabolism
/ Escherichia coli Proteins - metabolism
/ Humanities and Social Sciences
/ Lipids
/ Lipopolysaccharides - metabolism
/ Membrane Lipids - metabolism
/ Science
/ Thinning
