Journal Article

Lsr2 acts as a cyclic di-GMP receptor that promotes keto-mycolic acid synthesis and biofilm formation in mycobacteria

Hu, Lihua, Li, Danting, Li, Weihui, Zhang, Hongyun, Xiang, Yulin, Ling, Xiaocui, Liu, Xiao, Guo, Minhao, Wang, Kun, Ou, Yanzhe, Zheng, Jiachen

2024

Overview

Cyclic di-GMP (c-di-GMP) is a second messenger that promotes biofilm formation in several bacterial species, but the mechanisms are often unclear. Here, we report that c-di-GMP promotes biofilm formation in mycobacteria in a manner dependent on the nucleoid-associated protein Lsr2. We show that c-di-GMP specifically binds to Lsr2 at a ratio of 1:1. Lsr2 upregulates the expression of HadD, a (3R)-hydroxyacyl-ACP dehydratase, thus promoting the synthesis of keto-mycolic acid and biofilm formation. Thus, Lsr2 acts as a c-di-GMP receptor that links the second messenger’s function to lipid synthesis and biofilm formation in mycobacteria. Cyclic di-GMP (c-di-GMP) is a second messenger that promotes biofilm formation through unclear mechanisms in several bacterial species. Here, Ling et al. report that c-di-GMP promotes biofilm formation in mycobacteria by binding to protein Lsr2, which upregulates the synthesis of keto-mycolic acid and thus biofilm formation.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 140/58 / 147/135 / 38/15 / 38/22 / 38/39 / 38/44 / 38/47 / 38/77 / 38/88 / 38/90 / 631/326/41 / 631/326/46 / 631/326/88 / 82/80 / 82/83 / Adipogenesis / Bacteria / Biofilms / Cyclic GMP - analogs & derivatives / Dehydration / Genotype & phenotype / Humanities and Social Sciences / Keto Acids / Lipids / Morphology / Motility / multidisciplinary / Mycobacterium / Mycolic Acids / Proteins / Receptors / Science / Science (multidisciplinary) / Sedimentation & deposition / Synthesis / Tuberculosis