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Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivui
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Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivui
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Journal Article
Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivui
2024
Overview
Electrocatalytic carbon dioxide (CO
2
) reduction by CO
2
reductases is a promising approach for biomanufacturing. Among all known biological or chemical catalysts, hydrogen-dependent carbon dioxide reductase from
Thermoanaerobacter kivui
(
Tk
HDCR) possesses the highest activity toward CO
2
reduction. Herein, we engineer
Tk
HDCR to generate an electro-responsive carbon dioxide reductase considering the safety and convenience. To achieve this purpose, a recombinant
Escherichia coli Tk
HDCR overexpression system is established. The formate dehydrogenase is obtained via subunit truncation and rational design, which enables direct electron transfer (DET)-type bioelectrocatalysis with a near-zero overpotential. By applying a constant voltage of −500 mV (
vs
. SHE) to a mediated electrolytic cell, 22.8 ± 1.6 mM formate is synthesized in 16 h with an average production rate of 7.1 ± 0.5 μmol h
−1
cm
−2
, a Faradaic efficiency of 98.9% and a half-cell energy efficiency of 94.4%. This study provides an enzyme candidate for high efficient CO
2
reduction and opens up a way to develop paradigm for CO
2
-based bio-manufacturing.
Thermoanaerobacter kivui
-drived CO
2
reductase (
Tk
HDCR) requires hydrogen as substrate, which can lead to safety issue. Here, the authors engineered
Tk
HDCR into an electro-responsive carbon dioxide reductase to harvest electrons from either an external mediator or a polarized electroactive surface.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 82/80
/ 82/83
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ E coli
/ Escherichia coli - metabolism
/ Formate Dehydrogenases - genetics
/ Formate Dehydrogenases - metabolism
/ Humanities and Social Sciences
/ Hydrogen
/ Protein Engineering - methods
/ Recombinant Proteins - genetics
/ Recombinant Proteins - metabolism
/ Science
/ Thermoanaerobacter - enzymology
