Asset Details
Do you wish to reserve the book?
Identification of leukemia-enriched signature through the development of a comprehensive pediatric single-cell atlas
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Identification of leukemia-enriched signature through the development of a comprehensive pediatric single-cell atlas
Do you wish to request the book?
Identification of leukemia-enriched signature through the development of a comprehensive pediatric single-cell atlas
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Identification of leukemia-enriched signature through the development of a comprehensive pediatric single-cell atlas
2025
Overview
Single-cell transcriptome profiling enables unparalleled characterization of the heterogeneous microenvironment of pediatric leukemias. To facilitate comparative analyses and generate pediatric leukemia signatures, we collect, process, and annotate single-cell data comprising over 540,000 cells from 159 different pediatric acute leukemia (myeloid, lymphoid, mixed phenotype lineages) and healthy bone marrow (BM) samples, profiled in our lab and curated from publicly available studies. The analysis identifies a leukemia-enriched signature of nine genes with over-expression in leukemic blast compared to healthy BM cells. This signature is also consistently over-expressed in leukemia samples compared to normal BM in bulk RNA-seq datasets (over 2000 samples). Outcome-based analysis on diagnosis samples using measurable residual disease (MRD) status depicts a significant association of oncogene-induced senescence and g-protein activation pathways with MRD positivity. MRD positivity across pediatric leukemias is also correlated with significant depletion of CD8+ and CD4+ naïve T-cells and M1-macrophages at diagnosis. To enable easy access to this comprehensive pediatric leukemia single-cell atlas, we develop the Pediatric Single-cell Cancer Atlas (PedSCAtlas,
https://bhasinlab.bmi.emory.edu/PediatricSCAtlas/
). The atlas allows for quick exploration of single-cell data based on genes, cell type composition, and clinical outcomes to understand the cellular landscape of pediatric leukemias.
Single-cell transcriptomics can allow detailed analyses of paediatric leukaemias. Here, the authors integrate single-cell data from 159 paediatric leukaemia and healthy bone marrow samples, identify a leukaemia-enriched gene expression signature, and develop the integrative online Paediatric Single-cell Cancer Atlas to explore and analyse this harmonised dataset.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
