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Preliminary study on the freeze-drying of human bone marrow-derived mesenchymal stem cells
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Preliminary study on the freeze-drying of human bone marrow-derived mesenchymal stem cells
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Journal Article
Preliminary study on the freeze-drying of human bone marrow-derived mesenchymal stem cells
2010
Overview
Long-term preservation and easy transportation of human bone marrow-derived mesenchymal stem cells （hBM-MSCs） will facilitate their application in medical treatment and bioengineering. A pilot study on the freeze-drying of hBM-MSCs was carried out. hBM-MSCs were loaded with trehalose. The glass transition temperature of the freeze-drying suspension was measured to provide information for the cooling and primary drying experiment. After freeze-drying, various rehydration processes were tested. The highest recovery rate of hBM-MSCs was （69.33±13.08）%. Possible methods to improve freeze-drying outcomes are discussed. In conclusion, the present study has laid a foundation for the freeze-drying hBM-MSCs.
Publisher
SP Zhejiang University Press,Springer Nature B.V,Zhejiang University Press
Subject
/ Bone Marrow Cells - cytology
/ Bone Marrow Cells - metabolism
/ Bones
/ Cell Culture Techniques - methods
/ Drying
/ HBM
/ Human
/ Humans
/ Mesenchymal Stromal Cells - cytology
/ Mesenchymal Stromal Cells - metabolism
/ Pilots
/ Trehalose - pharmacokinetics
/ 冷冻干燥
/ 干燥试验
/ 温度测量
/ 玻璃化转变
/ 生物工程
/ 长期保存
/ 骨髓间充质干细胞
