Journal Article

NanoBRET binding assay for histamine H2 receptor ligands using live recombinant HEK293T cells

Grätz, Lukas, Bresinsky, Merlin, Müller, Christoph, Pockes, Steffen, Tropmann, Katharina, Bernhardt, Günther

2020

Overview

Fluorescence/luminescence-based techniques play an increasingly important role in the development of test systems for the characterization of future drug candidates, especially in terms of receptor binding in the field of G protein-coupled receptors (GPCRs). In this article, we present the establishment of a homogeneous live cell-based BRET binding assay for the histamine H 2 receptor with different fluorescently labeled squaramide-type compounds synthesized in the course of this study. Py-1-labeled ligand 8 (UR-KAT478) was found to be most suitable in BRET saturation binding experiments with respect to receptor affinity (p K d  = 7.35) and signal intensity. Real-time kinetic experiments showed a full association of 8 within approximately 30 min and a slow dissociation of the ligand from the receptor. Investigation of reference compounds in BRET-based competition binding with 8 yielded p K i values in agreement with radioligand binding data. This study shows that the BRET binding assay is a versatile test system for the characterization of putative new ligands at the histamine H 2 receptor and represents a valuable fluorescence-based alternative to canonical binding assays.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 631/1647 / 631/45 / 639/638 / Drug development / Fluorescence / G protein-coupled receptors / Histamine / Histamine H2 receptors / Humanities and Social Sciences / Ligands / multidisciplinary / Science / Science (multidisciplinary)