Asset Details
Do you wish to reserve the book?
Graphene Oxide Nanosheets Induce Mitochondrial Toxicity in Human Ovarian Granulosa Cells: Implications for Female Reproductive Health
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Graphene Oxide Nanosheets Induce Mitochondrial Toxicity in Human Ovarian Granulosa Cells: Implications for Female Reproductive Health
Do you wish to request the book?
Graphene Oxide Nanosheets Induce Mitochondrial Toxicity in Human Ovarian Granulosa Cells: Implications for Female Reproductive Health
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Graphene Oxide Nanosheets Induce Mitochondrial Toxicity in Human Ovarian Granulosa Cells: Implications for Female Reproductive Health
2025
Overview
Graphene oxide (GO) has promising biomedical applications, but its potential toxicity to the female reproductive system is underexplored. This study investigates the short-term effects of a single dose of GO nanosheets on human ovarian granulosa cells, focusing on mitochondrial damage.
First, cell viability was detected by CCK-8 and apoptosis was detected by flow cytometry to assess the cytotoxicity of GO on KGN. Second, reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and mitochondrial morphology were observed by confocal microscopy, mitochondrial and sub-mitochondrial structure by transmission electron microscopy (TEM), quantitative analysis of ATP and mitochondrial complex I enzyme activity by luminosity value and autophagy by flow cytometry to assess the mitochondrial toxicity of GO on KGN cells.
The 72h half-maximum effective concentration (EC50) value of GO was determined to be 29.73 μg/mL. GO induced cell death in a dose-dependent manner, with significant effects on cell viability even at low doses (1 μg/mL). Exposure to low GO concentrations resulted in abnormal mitochondrial morphology and function, including mitochondrial breakage, membrane damage, reduced mitochondrial cristae, enhanced autophagy, decreased ATP production, decreased MMP, and decreased enzymatic activity of mitochondrial complex I. Mitochondrial function returned to normal levels on day 7 after KGN cells left the GO-exposed environment.
This study demonstrates that short-term exposure to low-dose GO causes mitochondrial damage in human ovarian granulosa cells, highlighting the need for further research on the safety of GO, particularly regarding its potential effects on reproductive health. However, GO-induced transient mitochondrial damage is highly likely to negatively affect ovarian reserve function, which needs to be further verified in animal models.
Publisher
Dove Medical Press Limited,Taylor & Francis Ltd,Dove,Dove Medical Press
Subject
/ Cell Survival - drug effects
/ Cells
/ Female
/ Females
/ Granulosa Cells - drug effects
/ Granulosa Cells - metabolism
/ Graphene
/ Graphite
/ human ovarian granulosa cells
/ Humans
/ Membrane Potential, Mitochondrial - drug effects
/ Ovaries
/ Reactive Oxygen Species - metabolism
/ Toxicity
/ Women
