Journal Article

The SKI complex is a broad-spectrum, host-directed antiviral drug target for coronaviruses, influenza, and filoviruses

Logue, James, Weston, Stuart, Keller, Chloe, Yu, Wenbo, Frieman, Matthew B., Dyall, Julie, Liang, Janie, McGrath, Marisa E., Baracco, Lauren, Matthews, Krystal, Jahrling, Peter B., MacKerell, Alexander D., Holbrook, Michael R., Hensley, Lisa E.

2020

Overview

The SARS-CoV-2 pandemic has made it clear that we have a desperate need for antivirals. We present work that the mammalian SKI complex is a broad-spectrum, host-directed, antiviral drug target. Yeast suppressor screening was utilized to find a functional genetic interaction between proteins from influenza A virus (IAV) and Middle East respiratory syndrome coronavirus (MERS-CoV) with eukaryotic proteins that may be potential host factors involved in replication. This screening identified the SKI complex as a potential host factor for both viruses. In mammalian systems siRNA-mediated knockdown of SKI genes inhibited replication of IAV and MERS-CoV. In silico modeling and database screening identified a binding pocket on the SKI complex and compounds predicted to bind. Experimental assays of those compounds identified three chemical structures that were antiviral against IAV and MERS-CoV along with the filoviruses Ebola and Marburg and two further coronaviruses, SARS-CoV and SARS-CoV-2. The mechanism of antiviral activity is through inhibition of viral RNA production. This work defines the mammalian SKI complex as a broad-spectrum antiviral drug target and identifies lead compounds for further development.

• Publisher: National Academy of Sciences
• Subject: Antiviral activity / Antiviral agents / Antiviral Agents - pharmacology / Biological Sciences / Cell Line / Chemical compounds / Coronaviridae / Coronavirus - drug effects / Coronaviruses / COVID-19 / Filoviridae - drug effects / Genes, Suppressor / Host-Pathogen Interactions - drug effects / Influenza / Influenza A / Lead compounds / Mammals / Microbiology / Middle East respiratory syndrome / Models, Molecular / Molecular Targeted Therapy / Multiprotein Complexes - metabolism / Orthomyxoviridae - drug effects / Pandemics / Protein Binding / Proteins / Replication / Respiratory diseases / RNA, Small Interfering - metabolism / RNA, Viral - genetics / RNA, Viral - metabolism / Saccharomyces cerevisiae - genetics / Screening / Severe acute respiratory syndrome coronavirus 2 / siRNA / Target recognition / Therapeutic targets / Viral diseases / Viral Proteins - metabolism / Virus Replication - drug effects / Viruses / Yeast