Journal Article

Proteomics and Recurrence of Atrial Fibrillation: A Pilot Study Nested in the PREDIMAR Trial

Macías-Ruíz, Rosa, Goni, Leticia, López, Begoña, Ibañez Criado, Jose Luis, Martínez-González, Miguel A., Razquin, Cristina, Ravassa, Susana, Ruiz-Canela, Miguel, Almendral, Jesús, Castellanos, Eduardo, Fernandez-Irigoyen, Joaquín, Tercedor, Luis, Santamaría, Enrique, Ramos, Pablo, Barrio-López, María Teresa, García-Bolao, Ignacio, Ibañez Criado, Alicia

2025

Overview

Introduction: Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia worldwide. Although catheter ablation is the most efficacious therapy, relapses occur frequently (30%) in the first year after ablation. Novel biomarkers of recurrence are needed for a better prediction of recurrence and management of AF. In this pilot study, we aimed to analyze the baseline proteome of subjects included in a case-control study to find differential proteins associated with AF recurrence. Methods: Baseline serum proteomics (354 proteins) data from 16 cases (recurrent AF) and 17 controls (non-recurrent) were obtained using MS/MS analysis. A false discovery rate was performed using a nonlinear fitting method for the selection of proteins. Logistic regression models were used to further analyze the association between differentially expressed proteins and AF recurrence. Results: Ten proteins were differentially represented in cases vs. controls. Two were upregulated in the cases compared to the controls: keratin type I cytoskeletal 17 (Fold-change [FC] = 2.14; p = 0.017) and endoplasmic bifunctional protein (FC = 1.65; p = 0.032). Eight were downregulated in the cases compared to the controls: C4bpA (FC = 0.64; p = 0.006), coagulation factor XI (FC = 0.83; p = 0.011), CLIC1 (FC = 0.62; p = 0.017), haptoglobin (FC = 0.37; p = 0.021), Ig alpha-2 chain C region (FC = 0.49; p = 0.022), C4bpB (FC = 0.73; p = 0.028), N-acetylglucosamine-1-phosphotransferase subunit gamma (FC = 0.61; p = 0.033), and platelet glycoprotein Ib alpha chain (FC = 0.84; p = 0.038). Conclusion: This pilot study identifies ten differentially expressed serum proteins associated with AF recurrence, offering potential biomarkers for improved prediction and management.

• Publisher: S. Karger AG,Karger Publishers
• Subject: Ablation / Aged / Atrial Fibrillation - blood / Atrial Fibrillation - genetics / Atrial Fibrillation - metabolism / Biomarkers - blood / Blood Proteins / Cardiac arrhythmia / Case-Control Studies / Catheter Ablation / Catheters / Female / Humans / Hypertension / Male / Medical instruments / Middle Aged / Peptides / Pilot Projects / Prevention / Proteins / Proteome / Proteomics / Proteomics - methods / Recurrence / Regression analysis / Sample size / Software / Tandem Mass Spectrometry