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CRISPR/Cas9‐mediated genome editing for wheat grain quality improvement
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CRISPR/Cas9‐mediated genome editing for wheat grain quality improvement
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CRISPR/Cas9‐mediated genome editing for wheat grain quality improvement
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Journal Article
CRISPR/Cas9‐mediated genome editing for wheat grain quality improvement
2021
Overview
[...]we have successfully mutated waxy gene to generate glutinous wheat with lower amylose content. PPO catalyses phenols oxidation into dark-coloured products, a feature often undesirable for wheat end-use products. [...]developing wheat cultivars with low PPO activity has always been an important goal in wheat breeding. [...]new allelic variations of the target genes (pinb, waxy, ppo and psy) were created in Fielder through Agrobacterium-delivered CRISPR/Cas9 system. Furthermore, many of the mutants had segregated out the CRISPR/Cas9 transgene. [...]we had successfully obtained new wheat germplasms with improved grain quality in hardness, starch composition and dough colour.
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