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Enhancement of activity and selectivity of Candida rugosa lipase and Candida antarctica lipase A by bioimprinting and/or immobilization for application in the selective ethanolysis of fish oil
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Enhancement of activity and selectivity of Candida rugosa lipase and Candida antarctica lipase A by bioimprinting and/or immobilization for application in the selective ethanolysis of fish oil
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Enhancement of activity and selectivity of Candida rugosa lipase and Candida antarctica lipase A by bioimprinting and/or immobilization for application in the selective ethanolysis of fish oil
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Journal Article
Enhancement of activity and selectivity of Candida rugosa lipase and Candida antarctica lipase A by bioimprinting and/or immobilization for application in the selective ethanolysis of fish oil
2011
Overview
Candida rugosa lipase (CRL) and Candida antarctica lipase A (CALA) with improved activity and selectivity were prepared for use in organic solvent media. CRL bioimprinted with fatty acids exhibited eightfold enhanced transesterification activity in hexane. Combination of bioimprinting and coating with lecithin or with immobilization did not improve the activity further. CALA was immobilized with and without bioimprinting, none of which improved the activity. All modified lipases were tested for selective ethanolysis of fish oil to concentrate omega-3 polyunsaturated fatty acids (PUFA). None of the preparations, except the immobilized ones catalysed ethanolysis. Immobilized CRL-catalyzed ethanolysis giving 27% (v/v) ethyl esters (EE) in 48 h, of which 43 mol% was oleic acid but no PUFA was detected in the EE fraction. Fatty acid selectivity of CALA was significantly improved by immobilization combined with bioimprinting, resulting in 5.5-fold lower omega-3 PUFA in EE.
Publisher
Springer-Verlag,Springer Netherlands,Springer,Springer Nature B.V
Subject
/ Bioconversions. Hemisynthesis
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Candida
/ coatings
/ Enzymes, Immobilized - chemistry
/ Enzymes, Immobilized - metabolism
/ Esters
/ Ethanol
/ Fatty Acids, Omega-3 - chemistry
/ Fatty Acids, Omega-3 - metabolism
/ Fatty Acids, Unsaturated - chemistry
/ Fatty Acids, Unsaturated - metabolism
/ Fish
/ Fundamental and applied biological sciences. Psychology
/ Lipase
/ methods
/ Methods. Procedures. Technologies
/ Molecular Imprinting - methods
/ Yeast
