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Long-term maintenance of human induced pluripotent stem cells by automated cell culture system
by
Iwata, Hiroo
, Suemori, Hirofumi
, Ando, Takeshi
, Yamauchi, Toshiaki
, Konagaya, Shuhei
in
631/532/2064
/ 639/166/985
/ 692/308/2171
/ Automation
/ Cell culture
/ Cell Culture Techniques - instrumentation
/ Cell Culture Techniques - methods
/ Cell Differentiation
/ Cells, Cultured
/ Dopamine receptors
/ Embryo cells
/ Flow Cytometry
/ Humanities and Social Sciences
/ Humans
/ Immunohistochemistry
/ Induced Pluripotent Stem Cells - cytology
/ Induced Pluripotent Stem Cells - metabolism
/ Karyotyping
/ multidisciplinary
/ Pancreas
/ Pluripotency
/ Science
/ Stem cells
/ Transcription Factors - genetics
/ Transcription Factors - metabolism
2015
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Long-term maintenance of human induced pluripotent stem cells by automated cell culture system
by
Iwata, Hiroo
, Suemori, Hirofumi
, Ando, Takeshi
, Yamauchi, Toshiaki
, Konagaya, Shuhei
in
631/532/2064
/ 639/166/985
/ 692/308/2171
/ Automation
/ Cell culture
/ Cell Culture Techniques - instrumentation
/ Cell Culture Techniques - methods
/ Cell Differentiation
/ Cells, Cultured
/ Dopamine receptors
/ Embryo cells
/ Flow Cytometry
/ Humanities and Social Sciences
/ Humans
/ Immunohistochemistry
/ Induced Pluripotent Stem Cells - cytology
/ Induced Pluripotent Stem Cells - metabolism
/ Karyotyping
/ multidisciplinary
/ Pancreas
/ Pluripotency
/ Science
/ Stem cells
/ Transcription Factors - genetics
/ Transcription Factors - metabolism
2015
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Long-term maintenance of human induced pluripotent stem cells by automated cell culture system
by
Iwata, Hiroo
, Suemori, Hirofumi
, Ando, Takeshi
, Yamauchi, Toshiaki
, Konagaya, Shuhei
in
631/532/2064
/ 639/166/985
/ 692/308/2171
/ Automation
/ Cell culture
/ Cell Culture Techniques - instrumentation
/ Cell Culture Techniques - methods
/ Cell Differentiation
/ Cells, Cultured
/ Dopamine receptors
/ Embryo cells
/ Flow Cytometry
/ Humanities and Social Sciences
/ Humans
/ Immunohistochemistry
/ Induced Pluripotent Stem Cells - cytology
/ Induced Pluripotent Stem Cells - metabolism
/ Karyotyping
/ multidisciplinary
/ Pancreas
/ Pluripotency
/ Science
/ Stem cells
/ Transcription Factors - genetics
/ Transcription Factors - metabolism
2015
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Long-term maintenance of human induced pluripotent stem cells by automated cell culture system
Journal Article
Long-term maintenance of human induced pluripotent stem cells by automated cell culture system
2015
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Overview
Pluripotent stem cells, such as embryonic stem cells and induced pluripotent stem (iPS) cells, are regarded as new sources for cell replacement therapy. These cells can unlimitedly expand under undifferentiated conditions and be differentiated into multiple cell types. Automated culture systems enable the large-scale production of cells. In addition to reducing the time and effort of researchers, an automated culture system improves the reproducibility of cell cultures. In the present study, we newly designed a fully automated cell culture system for human iPS maintenance. Using an automated culture system, hiPS cells maintained their undifferentiated state for 60 days. Automatically prepared hiPS cells had a potency of differentiation into three germ layer cells including dopaminergic neurons and pancreatic cells.
Publisher
Nature Publishing Group UK,Nature Publishing Group
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