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A Halloween gene noppera-bo encodes a glutathione S-transferase essential for ecdysteroid biosynthesis via regulating the behaviour of cholesterol in Drosophila
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A Halloween gene noppera-bo encodes a glutathione S-transferase essential for ecdysteroid biosynthesis via regulating the behaviour of cholesterol in Drosophila
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A Halloween gene noppera-bo encodes a glutathione S-transferase essential for ecdysteroid biosynthesis via regulating the behaviour of cholesterol in Drosophila
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Journal Article
A Halloween gene noppera-bo encodes a glutathione S-transferase essential for ecdysteroid biosynthesis via regulating the behaviour of cholesterol in Drosophila
2014
Overview
In insects, the precise timing of moulting and metamorphosis is strictly guided by ecdysteroids that are synthesised from dietary cholesterol in the prothoracic gland (PG). In the past decade, several ecdysteroidogenic enzymes, some of which are encoded by the Halloween genes, have been identified and characterised. Here, we report a novel Halloween gene,
noppera-bo
(
nobo
), that encodes a member of the glutathione
S
-transferase family.
nobo
was identified as a gene that is predominantly expressed in the PG of the fruit fly
Drosophila melanogaster
. We generated a
nobo
knock-out mutant, which displayed embryonic lethality and a naked cuticle structure. These phenotypes are typical for Halloween mutants showing embryonic ecdysteroid deficiency. In addition, the PG-specific
nobo
knock-down larvae displayed an arrested phenotype and reduced 20-hydroxyecdysone (20E) titres. Importantly, both embryonic and larval phenotypes were rescued by the administration of 20E or cholesterol. We also confirm that PG cells in
nobo
loss-of-function larvae abnormally accumulate cholesterol. Considering that cholesterol is the most upstream material for ecdysteroid biosynthesis in the PG, our results raise the possibility that
nobo
plays a crucial role in regulating the behaviour of cholesterol in steroid biosynthesis in insects.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 38/39
/ 38/70
/ 38/71
/ 38/77
/ 38/88
/ 38/89
/ 45/41
/ 64/24
/ Animals
/ Drosophila melanogaster - genetics
/ Drosophila melanogaster - growth & development
/ Drosophila melanogaster - metabolism
/ Drosophila Proteins - genetics
/ Embryos
/ Enzymes
/ Glutathione Transferase - genetics
/ Glutathione Transferase - isolation & purification
/ Humanities and Social Sciences
/ Insects
/ Larva - growth & development
/ Larvae
/ Metamorphosis, Biological - genetics
/ Molting
/ Science
