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Acetate oxidation by syntrophic association between Geobacter sulfurreducens and a hydrogen-utilizing exoelectrogen
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Acetate oxidation by syntrophic association between Geobacter sulfurreducens and a hydrogen-utilizing exoelectrogen
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Journal Article
Acetate oxidation by syntrophic association between Geobacter sulfurreducens and a hydrogen-utilizing exoelectrogen
2013
Overview
Anodic microbial communities in acetate-fed microbial fuel cells (MFCs) were analyzed using stable-isotope probing of 16S rRNA genes followed by denaturing gradient gel electrophoresis. The results revealed that
Geobacter sulfurreducens
and
Hydrogenophaga
sp. predominated in the anodic biofilm. Although the predominance of
Geobacter
sp. as acetoclastic exoelectrogens in acetate-fed MFC systems has been often reported, the ecophysiological role of
Hydrogenophaga
sp. is unknown. Therefore, we isolated and characterized a bacterium closely related to
Hydrogenophaga
sp. (designated strain AR20). The newly isolated strain AR20 could use molecular hydrogen (H
2
), but not acetate, with carbon electrode as the electron acceptor, indicating that the strain AR20 was a hydrogenotrophic exoelectrogen. This evidence raises a hypothesis that acetate was oxidized by
G. sulfurreducens
in syntrophic cooperation with the strain AR20 as a hydrogen-consuming partner in the acetate-fed MFC. To prove this hypothesis,
G. sulfurreducens
strain PCA was cocultivated with the strain AR20 in the acetate-fed MFC without any dissolved electron acceptors. In the coculture MFC of
G. sulfurreducens
and strain AR20, current generation and acetate degradation were the highest, and the growth of strain AR20 was observed. No current generation, acetate degradation and cell growth occurred in the strain AR20 pure culture MFC. These results show for the first time that
G. sulfurreducens
can oxidize acetate in syntrophic cooperation with the isolated
Hydrogenophaga
sp. strain AR20, with electrode as the electron acceptor.
Publisher
Nature Publishing Group UK,Oxford University Press,Nature Publishing Group
Subject
/ Bioelectric Energy Sources - microbiology
/ Biofilms
/ Biomedical and Life Sciences
/ Comamonadaceae - classification
/ Comamonadaceae - growth & development
/ Denaturing Gradient Gel Electrophoresis
/ Ecology
/ Geobacter - growth & development
/ Hydrogen
/ Microbial Genetics and Genomics
/ Original
/ RNA, Ribosomal, 16S - genetics
/ rRNA 16S
