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Controlled gene expression in primary Lgr5 organoid cultures
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Controlled gene expression in primary Lgr5 organoid cultures
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Journal Article
Controlled gene expression in primary Lgr5 organoid cultures
2012
Overview
The controlled overexpression or knockdown of gene expression in primary organoid cultures of mouse endodermal epithelia is described. This should enable
ex vivo
studies of mammalian gene function.
The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinase–inducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Animals
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Gene Knockdown Techniques - methods
/ Green Fluorescent Proteins - genetics
/ Humans
/ Intestinal Mucosa - cytology
/ Mammals
/ Mice
/ MicroRNA
/ Receptors, G-Protein-Coupled - physiology
/ Receptors, Notch - physiology
