Journal Article

Solubilized Amnion Membrane Hyaluronic Acid Hydrogel Accelerates Full‐Thickness Wound Healing

Mack, David L., Murphy, Sean V., Song, Lujie, Atala, Anthony, Skardal, Aleksander, Soker, Shay, Haug, Rebecca, Sutton, Khiry, Jackson, John

2017

Overview

The early and effective treatment of wounds is vital to ensure proper wound closure and healing with appropriate functional and cosmetic outcomes. The use of human amnion membranes for wound care has been shown to be safe and effective. However, the difficulty in handling and placing thin sheets of membrane, and the high costs associated with the use of living cellularized tissue has limited the clinical application of amniotic membrane wound healing products. Here, we describe a novel amnion membrane‐derived product, processed to result in a cell‐free solution, while maintaining high concentrations of cell‐derived cytokines and growth factors. The solubilized amnion membrane (SAM) combined with the carrier hyaluronic acid (HA) hydrogel (HA‐SAM) is easy to produce, store, and apply to wounds. We demonstrated the efficacy of HA‐SAM as a wound treatment using a full‐thickness murine wound model. HA‐SAM significantly accelerated wound closure through re‐epithelialization and prevented wound contraction. HA‐SAM‐treated wounds had thicker regenerated skin, increased total number of blood vessels, and greater numbers of proliferating keratinocytes within the epidermis. Overall, this study confirms the efficacy of the amnion membrane as a wound treatment/dressing, and overcomes many of the limitations associated with using fresh, cryopreserved, or dehydrated tissue by providing a hydrogel delivery system for SAM. Stem Cells Translational Medicine 2017;6:2020–2032 Time‐course images from in vivo wound healing study. A 2 × 2 cm full‐thickness wound was created on the back of nude mice and received one of three treatment options; (A): Untreated other than standard bandaging; (B): HA‐gel only or; (C): HA‐SAM gel.

• Publisher: Oxford University Press,John Wiley and Sons Inc
• Subject: Amnion / Amnion - chemistry / Amnion - metabolism / Amniotic membrane / Amniotic Stem Cells / Animals / Biomaterial / Blood vessels / Care and treatment / Cells, Cultured / Clinical trials / Contraction / Cryopreservation / Cytokines / Cytokines - metabolism / Diabetes / Epidermis / Female / Fibroblasts - cytology / Fibroblasts - physiology / Growth factors / Humans / Hyaluronic acid / Hyaluronic Acid - chemistry / Hydrogel / Hydrogels / Hydrogels - chemistry / Intercellular Signaling Peptides and Proteins - metabolism / Keratinocytes / Keratinocytes - cytology / Keratinocytes - physiology / Male / Mice / Mice, Nude / Re-Epithelialization / Skin / Solubilized amnion membrane / Stem cell transplantation / Stem cells / Studies / Tissue Engineering - methods / Tissue Engineering and Regenerative Medicine / Tissue Scaffolds - chemistry / Translational s and Reviews / Ulcers / Wound healing / Wound Healing / Fibrosis / Wounds and injuries