Journal Article

Impact of Mycotoxin Contaminations on Aquatic Organisms: Toxic Effect of Aflatoxin B1 and Fumonisin B1 Mixture

Arangia, Alessia, Crupi, Rosalia, Cuzzocrea, Salvatore, Peritore, Alessio Filippo, Di Paola, Davide, Iaria, Carmelo, Gugliandolo, Enrico, Spanò, Nunziacarla, Capparucci, Fabiano

2022

Overview

(1) Background: Multiple contaminations of several mycotoxins have been detected in human and veterinary food and feed worldwide. To date, a number of studies on the combined effects of mycotoxins have been conducted on cell and animal models, but very limited studies have been done on aquatic organisms. (2) The purpose of the present study was to evaluate the combined toxic effects of Aflatoxin B1 (AFB1) and Fumonisin B1 (FB1) on zebrafish (Danio rerio) embryos. (3) Results: Our results showed that the combination of AFB1 and FB1 at nontoxic concentrations exerted a negative effect on the lethal endpoints analyzed, such as survival, hatching, and heart rate. In addition, the mixture of mycotoxins caused an increase in the levels of enzymes and proteins involved in the antioxidant process, such as superoxide dismutase (SOD) and catalase (CAT), both in terms of protein levels and gene expression, as well as an increase in the levels of the detoxification enzymes glutathione s-transferases (GST) and cytochromes P450 (CYP450). Furthermore, we showed that the mycotoxin mixture induced an increase in pro-apoptotic proteins such as bax and caspase 3, and at the same time reduced the gene expression of the anti-apoptotic bcl-2 protein. Finally, a significant decrease in thyroid function was observed in terms of triiodothyronine (T3), thyroxine (T4), and vitellogenin (VTG) levels. (4) Conclusion: We can say that the mixture of mycotoxins carries a greater risk factor than individual presences. There is a greater need for effective detoxification methods to control and reduce the toxicity of multiple mycotoxins and reduce the toxicity of multiple mycotoxins in feed and throughout the food chain.

• Publisher: MDPI AG,MDPI
• Subject: Aflatoxin B1 / Aflatoxin B1 - toxicity / Aflatoxins / Animal models / Animals / Apoptosis / Aquatic animals / Aquatic animals, Effect of water pollution on / Aquatic Organisms / BAX protein / Bcl-2 protein / Caspase-3 / Catalase / Cell culture / combined toxicity / Control methods / Cytochromes P450 / Danio rerio / Detoxification / Edema / Embryos / Environmental aspects / Enzymes / Food chains / Food contamination & poisoning / Fumonisin B1 / Fumonisins - toxicity / Gene expression / Genes / Glutathione / Hatching / Health aspects / Heart rate / Humans / Italy / Metabolism / Metabolites / Mixtures / Morphology / Mycotoxins / Mycotoxins - toxicity / Oxidative stress / Proteins / Risk analysis / Risk factors / Scoliosis / Superoxide dismutase / Thyroid / Thyroid gland / Thyroid hormones / Thyroxine / Toxicity / Water pollution / Zebrafish