Journal Article

Lactobacillus sakei suppresses collagen-induced arthritis and modulates the differentiation of T helper 17 cells and regulatory B cells

Min, Hong Ki, Choi, Jeong Won, Ryu, Jun-Geol, Na, Hyun Sik, Ji, Geun Eog, Park, Sung-Hwan, Park, Myeong Soo, Cho, Mi-La, Lee, Seung Yoon, Jung, Yunju, Lee, Seon-Yeong, Park, Sang-Jun, Kwon, Bin, Ryu, Jaeyoon, Jhun, Jooyeon

2020

Overview

Background To evaluate the immunomodulatory effect of Lactobacillus sakei in a mouse model of rheumatoid arthritis (RA) and in human immune cells. Methods We evaluated whether L. sakei reduced the severity of collagen-induced arthritis (CIA) and modulated interleukin (IL)-17 and IL-10 levels, as well as whether it affected the differentiation of CD4 + T cells and regulatory B cells. We evaluated osteoclastogenesis after culturing bone marrow-derived mononuclear cells with L. sakei. Results The differentiation of T helper 17 cells and the serum level of IL-17 were suppressed by L. sakei in both human peripheral blood mononuclear cells and mouse splenocytes. The serum level of IL-10 was significantly increased in the L. sakei -treated group, whereas the regulatory T cell population was unchanged. The population of regulatory B cells significantly increased the in L. sakei -treated group. Oral administration of L. sakei reduced the arthritis incidence and score in mice with CIA. Finally, osteoclastogenesis and the mRNA levels of osteoclast-related genes were suppressed in the L. sakei -treated group. Conclusion L. sakei exerted an anti-inflammatory effect in an animal model of RA, regulated Th17 and regulatory B cell differentiation, and suppressed osteoclastogenesis. Our findings suggest that L. sakei has therapeutic potential for RA.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Analysis / Animal models / Animals / Ankle / Arthritis / Arthritis, Experimental - therapy / B cells / B-Lymphocytes, Regulatory / Biomedical and Life Sciences / Biomedicine / Bone marrow / CD4 antigen / Cell Differentiation / Collagen / Cytokines / Edema / Enzymes / Erythema / Government agencies / Helper cells / Immunobiology and immunotherapy / Immunomodulation / Immunomodulators / Inflammation / Interleukin 10 / Interleukin 17 / Interleukins / Laboratory animals / Lactobacillus sakei / Leukocytes (mononuclear) / Lymphocytes B / Lymphocytes T / Medicine/Public Health / Mice / Microbiome / mRNA / Oral administration / Osteoclastogenesis / Pathogenesis / Peripheral blood mononuclear cells / Probiotics / R&D / Regulatory B cell / Research & development / Rheumatoid arthritis / Rheumatoid factor / Splenocytes / T cells / T helper 17 cell / T-Lymphocytes, Regulatory / Th17 Cells