Journal Article

Aldaulactone – An Original Phytotoxic Secondary Metabolite Involved in the Aggressiveness of Alternaria dauci on Carrot

Poupard, Pascal, Berruyer, Romain, Courtial, Julia, Hamama, Latifa, Ogé, Laurent, Hamon, Bruno, Guichard, Esteban, Voisine, Linda, Boureau, Tristan, Yovanopoulos, Claire, Renaux, Yann, Helesbeux, Jean-Jacques, Briard, Mathilde, Richomme, Pascal, Lecomte, Mickaël, Gagné, Séverine

2018

Overview

Qualitative plant resistance mechanisms and pathogen virulence have been extensively studied since the formulation of the gene-for-gene hypothesis. The mechanisms involved in the quantitative traits of aggressiveness and plant partial resistance are less well-known. Nevertheless, they are prevalent in most plant-necrotrophic pathogen interactions, including the - interaction. Phytotoxic metabolite production by the pathogen plays a key role in aggressiveness in these interactions. The aim of the present study was to explore the link between aggressiveness and toxin production. We challenged carrot embryogenic cell cultures from a susceptible genotype (H1) and two partially resistant genotypes (I2 and K3) with exudates from strains with various aggressiveness levels. Interestingly, -resistant carrot genotypes were only affected by exudates from the most aggressive strain in our study (ITA002). Our results highlight a positive link between aggressiveness and the fungal exudate cell toxicity. We hypothesize that the fungal exudate toxicity was linked with the amount of toxic compounds produced by the fungus. Interestingly, organic exudate production by the fungus was correlated with aggressiveness. Hence, we further analyzed the fungal organic extract using HPLC, and correlations between the observed peak intensities and fungal aggressiveness were measured. One observed peak was closely correlated with fungal aggressiveness. We succeeded in purifying this peak and NMR analysis revealed that the purified compound was a novel 10-membered benzenediol lactone, a polyketid that we named 'aldaulactone'. We used a new automated image analysis method and found that aldaulactone was toxic to cultured plant cells at those concentrations. The effects of both aldaulactone and fungal organic extracts were weaker on I2-resistant carrot cells compared to H1 carrot cells. Taken together, our results suggest that: (i) aldaulactone is a new phytotoxin, (ii) there is a relationship between the amount of aldaulactone produced and fungal aggressiveness, and (iii) carrot resistance to involves mechanisms of resistance to aldaulactone.

• Publisher: Frontiers Media SA,Frontiers,Frontiers Media S.A
• Subject: Aggressiveness / Alternaria dauci / Alternaria leaf blight / Carrots / Correlation / Determinism / Exudates / Exudation / fungal pathogenicity / Fungi / Genotypes / Image analysis / Image processing / in vitro culture / Life Sciences / Metabolites / NMR / Nuclear magnetic resonance / Pathogens / Phytopathology and phytopharmacy / phytotoxin / Phytotoxins / Plant cells / Plant resistance / Plant Science / quantitative disease resistance / Signal transduction / Solvents / Toxicity / Toxins / Vegetal Biology / Virulence