Journal Article

Natural Variation in Arabidopsis Cvi-0 Accession Reveals an Important Role of MPK12 in Guard Cell CO2 Signaling

Maran, Uko, Kangasjärvi, Saijaliisa, Pechter, Priit, Wang, Yuh-Shuh, Vaahtera, Lauri, Xu, Yang, Schroeder, Julian I., Gerst Talas, Ulvi, Wang, Cun, Kollist, Hannes, Roelfsema, M. Rob G., Loog, Mart, Brosché, Mikael, Hõrak, Hanna, Hu, Honghong, Valk, Ervin, Sindarovska, Yana, Jakobson, Liina, Kangasjärvi, Jaakko, Xiao, Chuanlei, Remm, Maido, García-Sosa, Alfonso T., Nuhkat, Maris, Tõldsepp, Kadri, Tang, Jing

2016

Overview

Plant gas exchange is regulated by guard cells that form stomatal pores. Stomatal adjustments are crucial for plant survival; they regulate uptake of CO2 for photosynthesis, loss of water, and entrance of air pollutants such as ozone. We mapped ozone hypersensitivity, more open stomata, and stomatal CO2-insensitivity phenotypes of the Arabidopsis thaliana accession Cvi-0 to a single amino acid substitution in MITOGEN-ACTIVATED PROTEIN (MAP) KINASE 12 (MPK12). In parallel, we showed that stomatal CO2-insensitivity phenotypes of a mutant cis (CO2-insensitive) were caused by a deletion of MPK12. Lack of MPK12 impaired bicarbonate-induced activation of S-type anion channels. We demonstrated that MPK12 interacted with the protein kinase HIGH LEAF TEMPERATURE 1 (HT1)-a central node in guard cell CO2 signaling-and that MPK12 functions as an inhibitor of HT1. These data provide a new function for plant MPKs as protein kinase inhibitors and suggest a mechanism through which guard cell CO2 signaling controls plant water management.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Air pollution / Amino acids / Arabidopsis / Arabidopsis - genetics / Arabidopsis - physiology / Arabidopsis Proteins - genetics / Arabidopsis Proteins - metabolism / Arabidopsis thaliana / Biology and Life Sciences / Carbon dioxide / Carbon Dioxide - metabolism / Chromosome Mapping / Colleges & universities / Developmental biology / Earth Sciences / Ecology and Environmental Sciences / Editing / Experiments / Funding / Gas exchange / Genetic Variation / Guards / Hypersensitivity / Kinases / Leaves / Life sciences / Mitogen-Activated Protein Kinases - metabolism / Ozone / Ozone - metabolism / Photosynthesis / Physical Sciences / Plant biology / Plant sciences / Pollutants / Proteins / Quantitative Trait Loci / Research and Analysis Methods / Roles / Signal Transduction / Stomata / Supervision / Water / Water management / Writing / Yeast