Journal Article

Homologous Boosting with Adenoviral Serotype 5 HIV Vaccine (rAd5) Vector Can Boost Antibody Responses despite Preexisting Vector-Specific Immunity in a Randomized Phase I Clinical Trial

Sarwar, Uzma N., Koup, Richard A., Nason, Martha C., Graham, Barney S., Ledgerwood, Julie E., Novik, Laura, Enama, Mary E., Bailer, Robert T., McDermott, Adrian B., Plummer, Sarah A., Mascola, John R., Roederer, Mario

2014

Overview

Needle-free delivery improves the immunogenicity of DNA vaccines but is also associated with more local reactogenicity. Here we report the first comparison of Biojector and needle administration of a candidate rAd5 HIV vaccine. Thirty-one adults, 18-55 years, 20 naive and 11 prior rAd5 vaccine recipients were randomized to receive single rAd5 vaccine via needle or Biojector IM injection at 1010 PU in a Phase I open label clinical trial. Solicited reactogenicity was collected for 5 days; clinical safety and immunogenicity follow-up was continued for 24 weeks. Overall, injections by either method were well tolerated. There were no serious adverse events. Frequency of any local reactogenicity was 16/16 (100%) for Biojector compared to 11/15 (73%) for needle injections. There was no difference in HIV Env-specific antibody response between Biojector and needle delivery. Env-specific antibody responses were more than 10-fold higher in subjects receiving a booster dose of rAd5 vaccine than after a single dose delivered by either method regardless of interval between prime and boost. Biojector delivery did not improve antibody responses to the rAd5 vaccine compared to needle administration. Homologous boosting with rAd5 gene-based vectors can boost insert-specific antibody responses despite pre-existing vector-specific immunity. Clinicaltrials.gov NCT00709605 NCT00709605.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Acquired immune deficiency syndrome / Adenoviridae - genetics / Adenoviruses / Adolescent / Adult / Adults / AIDS / AIDS Vaccines - immunology / Antibody response / Antigens / Biology and Life Sciences / Clinical trials / Cytotoxicity / Dengue fever / Deoxyribonucleic acid / DNA / DNA vaccines / Drug delivery systems / Enzyme-Linked Immunosorbent Assay / Genetic Vectors / Hepatitis / HIV / HIV Antibodies - biosynthesis / Homology / Human immunodeficiency virus / Humans / Immunity / Immunogenicity / Infections / Infectious diseases / Influenza / Medicine and health sciences / Middle Aged / Rad5 gene / Randomization / Research and Analysis Methods / Vaccines / West Nile virus / Young Adult