Journal Article

Community recommendations on cryoEM data archiving and validation

Rosenthal, Peter B., Topf, Maya, Schroeder, Gunnar F., Pye, Ryan, Istrate, Andrei, Patwardhan, Ardan, Lawson, Catherine L., Westbrook, John D., Bricogne, Gérard, Heymann, J. Bernard, Ganesan, Sai J., Kurisu, Genji, Kleywegt, Gerard J., Baldwin, Philip R., Abbott, Sanja, Huiskonen, Juha T., Danev, Radostin, Henderson, Richard, Warshamanage, Rangana, Scheres, Sjors H. W., Winn, Martyn D., Burley, Stephen K., Subramaniam, Sriram, Berrisford, John M., Pintilie, Grigore D., Rohou, Alexis, Wang, Zhe, Salih, Osman, Sachse, Carsten, Adams, Paul D., Hoch, Jeffrey C., Butcher, Sarah J., Murshudov, Garib N., Stagg, Scott M., Lander, Gabriel C., Young, Jasmine Y., Gutmanas, Aleksandras, Lok, Shee-Mei, Ludtke, Steven J., Morris, Kyle, Richardson, Jane S., Velankar, Sameer, Grant, Timothy, Kato, Takayuki, Croll, Tristan I., Sorzano, Carlos Oscar S., Choudhary, Preeti

2024

Overview

In January 2020, a workshop was held at EMBL-EBI (Hinxton, UK) to discuss data requirements for the deposition and validation of cryoEM structures, with a focus on single-particle analysis. The meeting was attended by 47 experts in data processing, model building and refinement, validation, and archiving of such structures. This report describes the workshop's motivation and history, the topics discussed, and the resulting consensus recommendations. Some challenges for future methods-development efforts in this area are also highlighted, as is the implementation to date of some of the recommendations.

• Publisher: International Union of Crystallography
• Subject: Archives & records / Archiving / Bioinformatics / Biological products / Collaboration / Conferences, meetings and seminars / Consortia / Cryoelectron Microscopy - methods / cryogenic-specimen electron microscopy / Crystallography / data archiving / Data Curation / Data processing / electron microscopy data bank / KNOWLEDGE MANAGEMENT AND PRESERVATION / Laboratories / Metadata / Microscopy / Molecular biology / protein data bank / Proteins / Quality control / Research Papers / Richardson, Jane S / single-particle cryoEM / Software / structure determination / validation / Workshops