Journal Article

Isospora and Lankesterella parasites (Eimeriidae, Apicomplexa) of passeriform birds in Europe: Infection rates, phylogeny, and pathogenicity

Šujanová, Alžbeta, Keckeisen, Carina, Himmel, Tanja, Chagas, Carolina R. F, Harl, Josef, Matt, Julia, Nedorost, Nora, Weissenböck, Herbert

2024

Overview

Wild birds are common hosts to numerous intracellular parasites such as single-celled eukaryotes of the family Eimeriidae (order Eucoccidiorida, phylum Apicomplexa). We investigated the infection rates, phylogeny, and pathogenicity of Isospora and Lankesterella parasites in wild and captive passerine birds. Blood and tissue samples of 815 wild and 15 deceased captive birds from Europe were tested using polymerase chain reaction and partial sequencing of the mitochondrial cytochrome b and cytochrome c oxidase I and the nuclear 18S rRNA gene. The infection rate for Lankesterella in wild birds was 10.7% compared to 5.8% for Isospora. Chromogenic in situ hybridization with probes targeting the parasites’ 18S rRNA was employed to identify the parasites’ presence in multiple organs, and hematoxylin–eosin staining was performed to visualize the parasite stages and assess associated lesions. Isospora parasites were mainly identified in the intestine, spleen, and liver. Extraintestinal tissue stages of Isospora were accompanied by predominantly lymphohistiocytic inflammation of varying severity. Lankesterella was most frequently detected in the spleen, lung, and brain; however, infected birds presented only a low parasite burden without associated pathological changes. These findings contribute to our understanding of Isospora and Lankesterella parasites in wild birds.

• Publisher: MDPI AG,MDPI
• Subject: Animals / Animals, Wild - parasitology / Apicomplexa / Bird Diseases - epidemiology / Bird Diseases - parasitology / Bird Diseases - pathology / Birds / blood / brain / chromogenic in situ hybridization / Coccidiosis - parasitology / Coccidiosis - veterinary / Cytochrome / Cytochrome b / Cytochrome-c oxidase / Cytochromes / Cytochromes b - genetics / DNA, Protozoan - genetics / Eimeriidae / Eimeriidae - genetics / Eimeriidae - pathogenicity / Eukaryotes / eukaryotic cells / Europe / Europe - epidemiology / family / genes / Hybridization / Infections / inflammation / Intestine / intestines / Isospora / Isospora - genetics / Isospora - isolation & purification / Isospora - pathogenicity / Isosporiasis - epidemiology / Isosporiasis - parasitology / Isosporiasis - veterinary / Lankesterella / Leukocytes / Liver / lungs / mitochondria / Musculoskeletal system / oxidoreductases / Parasites / Passeriformes / Passeriformes - parasitology / Pathogenicity / Pathogens / Pathology / Phylogeny / Polymerase chain reaction / Reptiles & amphibians / RNA, Ribosomal, 18S - genetics / rRNA 18S / Spleen / tissue stages / Veterinary medicine / wild birds