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Evaluation of In-house Genotyping Assay Performance Using Dried Blood Spot Specimens in the Global World Health Organization Laboratory Network
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Evaluation of In-house Genotyping Assay Performance Using Dried Blood Spot Specimens in the Global World Health Organization Laboratory Network
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Journal Article
Evaluation of In-house Genotyping Assay Performance Using Dried Blood Spot Specimens in the Global World Health Organization Laboratory Network
2012
Overview
In resource-limited settings, there is increased demand for human immunodeficiency virus type 1 drug resistance testing. Because preservation of plasma specimens is often not feasible in resource-limited settings, use of dried blood spots (DBSs) is being adopted. We used 2 panels of DBSs for genotyping assay validation and proficiency testing in selected laboratories in the World Health Organization laboratory network in 14 countries. An amplification sensitivity of 1000 copies/mL was achieved by 2 laboratories. Reproducibility and accuracy of nucleotide sequence determination and resistance-associated mutation identification from DBSs was similar to that previously determined for plasma. International shipping at ambient temperature had no significant effect on amplification success. These studies indicate that DBS-based genotyping is equally reproducible and reliable, although slightly less sensitive, compared with plasma.
Publisher
Oxford University Press,Oxford University Press (OUP)
Subject
/ Assays
/ Blood
/ Codons
/ Dried Blood Spot Testing - methods
/ Dried Blood Spot Testing - standards
/ Drug Resistance, Viral - genetics
/ Genotype
/ Genotyping Techniques - methods
/ Genotyping Techniques - standards
/ HIV
/ HIV 1
/ Human immunodeficiency virus
/ Human immunodeficiency virus 1
/ Humans
/ Mutation
/ Plasma
/ RNA
