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The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals
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The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals
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Journal Article
The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals
2017
Overview
Here the authors provide an extension of their original FLOTAC protocol, describing the Mini-FLOTAC technique, optimized to perform diagnosis of helminth and protozoan infections in humans and animals where centrifugation may not be practical.
This protocol is an extension to:
Nat. Protoc.5, 503–515 (2010); doi: 10.1038/nprot.2009.235; published online 25 February 2010
The FLOTAC is a sensitive, accurate, and precise technique for the diagnosis of protozoan and helminth infections in humans and animals. However, it requires centrifugation, and hence might be out of reach in resource-constrained settings. As an extension of the original FLOTAC protocol, this protocol describes the Mini-FLOTAC technique, a logical evolution of FLOTAC conceived to perform multivalent, qualitative, and quantitative diagnosis of helminth and protozoan infections in human and animal feces, and urine. This has been found to be of most use in the processing of large numbers of samples with rapid laboratory workup, and for veterinary applications directly on-farm. In addition to the Mini-FLOTAC apparatus, we describe the use of the Fill-FLOTAC, a closed system used to facilitate the performance of the first four consecutive steps of the Mini-FLOTAC technique: fecal sample collection and weighing, homogenization, filtration, and filling of the Mini-FLOTAC chambers. Processing of an individual sample using this protocol requires ∼12 min.
Publisher
Nature Publishing Group UK,Nature Publishing Group
