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Overexpression of a chloroplast-localized small heat shock protein OsHSP26 confers enhanced tolerance against oxidative and heat stresses in tall fescue
by
Sharmin, Shamima Akhtar
, Lee, Byung-Hyun
, Kim, Yong-Goo
, Lee, Ki-Won
, Lee, Sang-Hoon
, Kim, Kyung-Hee
, Alam, Iftekhar
in
Applied Microbiology
/ Biochemistry
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Biotechnology
/ Chloroplasts
/ Chloroplasts - genetics
/ Chloroplasts - metabolism
/ complementary DNA
/ drug effects
/ Electrolyte leakage
/ Encoding
/ enzymology
/ Festuca
/ Festuca - drug effects
/ Festuca - genetics
/ Festuca - physiology
/ Festuca - radiation effects
/ Festuca arundinacea
/ Fundamental and applied biological sciences. Psychology
/ Gene Expression
/ genes
/ genetics
/ Grasses
/ heat
/ Heat shock proteins
/ Heat stress
/ Heat tolerance
/ Heat-Shock Proteins, Small
/ Heat-Shock Proteins, Small - genetics
/ Heat-Shock Proteins, Small - metabolism
/ Hot Temperature
/ Life Sciences
/ metabolism
/ Microbiology
/ Original Research Paper
/ Oryza
/ Oryza - enzymology
/ Oryza - genetics
/ Oryza sativa
/ Oxidative Stress
/ paraquat
/ Photochemicals
/ Photosynthesis
/ photosystem II
/ Photosystem II Protein Complex
/ Photosystem II Protein Complex - metabolism
/ physiology
/ Plants (organisms)
/ Plants, Genetically Modified
/ polymerase chain reaction
/ radiation effects
/ rice
/ Stress response
/ stress tolerance
/ Stress, Physiological
/ Stresses
/ thiobarbituric acid-reactive substances
/ Tolerances
/ Transformations
/ Transgenic
/ Transgenic plants
2012
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Overexpression of a chloroplast-localized small heat shock protein OsHSP26 confers enhanced tolerance against oxidative and heat stresses in tall fescue
by
Sharmin, Shamima Akhtar
, Lee, Byung-Hyun
, Kim, Yong-Goo
, Lee, Ki-Won
, Lee, Sang-Hoon
, Kim, Kyung-Hee
, Alam, Iftekhar
in
Applied Microbiology
/ Biochemistry
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Biotechnology
/ Chloroplasts
/ Chloroplasts - genetics
/ Chloroplasts - metabolism
/ complementary DNA
/ drug effects
/ Electrolyte leakage
/ Encoding
/ enzymology
/ Festuca
/ Festuca - drug effects
/ Festuca - genetics
/ Festuca - physiology
/ Festuca - radiation effects
/ Festuca arundinacea
/ Fundamental and applied biological sciences. Psychology
/ Gene Expression
/ genes
/ genetics
/ Grasses
/ heat
/ Heat shock proteins
/ Heat stress
/ Heat tolerance
/ Heat-Shock Proteins, Small
/ Heat-Shock Proteins, Small - genetics
/ Heat-Shock Proteins, Small - metabolism
/ Hot Temperature
/ Life Sciences
/ metabolism
/ Microbiology
/ Original Research Paper
/ Oryza
/ Oryza - enzymology
/ Oryza - genetics
/ Oryza sativa
/ Oxidative Stress
/ paraquat
/ Photochemicals
/ Photosynthesis
/ photosystem II
/ Photosystem II Protein Complex
/ Photosystem II Protein Complex - metabolism
/ physiology
/ Plants (organisms)
/ Plants, Genetically Modified
/ polymerase chain reaction
/ radiation effects
/ rice
/ Stress response
/ stress tolerance
/ Stress, Physiological
/ Stresses
/ thiobarbituric acid-reactive substances
/ Tolerances
/ Transformations
/ Transgenic
/ Transgenic plants
2012
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Overexpression of a chloroplast-localized small heat shock protein OsHSP26 confers enhanced tolerance against oxidative and heat stresses in tall fescue
by
Sharmin, Shamima Akhtar
, Lee, Byung-Hyun
, Kim, Yong-Goo
, Lee, Ki-Won
, Lee, Sang-Hoon
, Kim, Kyung-Hee
, Alam, Iftekhar
in
Applied Microbiology
/ Biochemistry
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Biotechnology
/ Chloroplasts
/ Chloroplasts - genetics
/ Chloroplasts - metabolism
/ complementary DNA
/ drug effects
/ Electrolyte leakage
/ Encoding
/ enzymology
/ Festuca
/ Festuca - drug effects
/ Festuca - genetics
/ Festuca - physiology
/ Festuca - radiation effects
/ Festuca arundinacea
/ Fundamental and applied biological sciences. Psychology
/ Gene Expression
/ genes
/ genetics
/ Grasses
/ heat
/ Heat shock proteins
/ Heat stress
/ Heat tolerance
/ Heat-Shock Proteins, Small
/ Heat-Shock Proteins, Small - genetics
/ Heat-Shock Proteins, Small - metabolism
/ Hot Temperature
/ Life Sciences
/ metabolism
/ Microbiology
/ Original Research Paper
/ Oryza
/ Oryza - enzymology
/ Oryza - genetics
/ Oryza sativa
/ Oxidative Stress
/ paraquat
/ Photochemicals
/ Photosynthesis
/ photosystem II
/ Photosystem II Protein Complex
/ Photosystem II Protein Complex - metabolism
/ physiology
/ Plants (organisms)
/ Plants, Genetically Modified
/ polymerase chain reaction
/ radiation effects
/ rice
/ Stress response
/ stress tolerance
/ Stress, Physiological
/ Stresses
/ thiobarbituric acid-reactive substances
/ Tolerances
/ Transformations
/ Transgenic
/ Transgenic plants
2012
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Overexpression of a chloroplast-localized small heat shock protein OsHSP26 confers enhanced tolerance against oxidative and heat stresses in tall fescue
Journal Article
Overexpression of a chloroplast-localized small heat shock protein OsHSP26 confers enhanced tolerance against oxidative and heat stresses in tall fescue
2012
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Overview
Small heat shock proteins are involved in stress tolerance. We previously isolated and characterized a rice cDNA clone,
Oshsp26
, encoding a chloroplast-localized small heat shock protein that is expressed following oxidative or heat stress. In this study, we transferred this gene to tall fescue plants by an
Agrobacterium
-mediated transformation system. The integration and expression of the transgene was confirmed by PCR, Southern, northern, and immunoblot analyzes. Compared to the control plants, the transgenic plants had significantly lower electrolyte leakage and accumulation of thiobarbituric acid-reactive substances when exposed to heat or methyl viologen. The photochemical efficiency of photosystem II (PSII) (Fv/Fm) in the transgenic tall fescue plants was higher than that in the control plants during heat stress (42°C). These results suggest that the OsHSP26 protein plays an important role in the protection of PSII during heat and oxidative stress in vivo.
Publisher
Springer Netherlands,Springer,Springer Nature B.V
Subject
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Encoding
/ Festuca
/ Fundamental and applied biological sciences. Psychology
/ genes
/ genetics
/ Grasses
/ heat
/ Heat-Shock Proteins, Small - genetics
/ Heat-Shock Proteins, Small - metabolism
/ Oryza
/ paraquat
/ Photosystem II Protein Complex
/ Photosystem II Protein Complex - metabolism
/ Plants, Genetically Modified
/ rice
/ Stresses
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