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Development of a new monoclonal antibody specific to mouse Vγ6 chain
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Development of a new monoclonal antibody specific to mouse Vγ6 chain
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Development of a new monoclonal antibody specific to mouse Vγ6 chain
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Journal Article
Development of a new monoclonal antibody specific to mouse Vγ6 chain
2019
Overview
There are seven Vγ gene segments in the TCR γ chain loci of mice. We developed monoclonal antibodies (mAbs) specific to the Vγ6 chain (Heilig & Tonegawa nomenclature). By immunizing Vγ4/6 KO mice with complementarity-determining region peptides in Vγ6 chains, we generated three hybridomas. These hybridomas produced mAbs capable of cell surface staining of Vγ6/Vδ1 gene–transfected T-cell line lacking TCR as well as of Vγ1 − Vγ4 − Vγ5 − Vγ7 − γδ T cells and the CD3 high TCRδ int γδ T cells in various organs. The location of Vγ6 + γδ T cells, which peaked in the newborn thymus, was associated with mTEC. In vivo administration of clone 1C10-1F7 mAb impaired protection against Klebsiella pneumoniae infection but ameliorated psoriasis-like dermatitis induced by imiquimod treatment. These new mAbs are useful to elucidate the development, location, and functions of Vγ6 γδ T cells in mice.
Publisher
Life Science Alliance LLC
Subject
/ Antibodies, Monoclonal - immunology
/ Antibodies, Monoclonal - pharmacology
/ Antibody Formation - immunology
/ Antibody Specificity - immunology
/ Female
/ Mice
/ Receptors, Antigen, T-Cell, gamma-delta - antagonists & inhibitors
/ Receptors, Antigen, T-Cell, gamma-delta - immunology
/ Receptors, Antigen, T-Cell, gamma-delta - metabolism
/ Resource
