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SARS-CoV-2 and the COVID-19 disease: a mini review on diagnostic methods
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SARS-CoV-2 and the COVID-19 disease: a mini review on diagnostic methods
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Journal Article
SARS-CoV-2 and the COVID-19 disease: a mini review on diagnostic methods
2020
Overview
Coronavirus disease 2019 (COVID-19) is an infectious disease initially reported in China and currently worldwide dispersed caused by a new coronavirus (SARS-CoV-2 or 2019-nCoV) affecting more than seven million people around the world causing more than 400 thousand deaths (on June 8th, 2020). The diagnosis of COVID-19 is based on the clinical and epidemiological history of the patient. However, the gold standard for COVID-19 diagnosis is the viral detection through the amplification of nucleic acids. Although the quantitative Reverse-Transcription Polymerase Chain Reaction (RT-PCR) has been described as the gold standard for diagnosing COVID-19, there are several difficulties involving its use. Here we comment on RT-PCR and describe alternative tests developed for the diagnosis of COVID-19.
Publisher
Instituto de Medicina Tropical de Sao Paulo,Instituto de Medicina Tropical,Instituto de Medicina Tropical de São Paulo,Universidade de São Paulo (USP)
Subject
/ Betacoronavirus - isolation & purification
/ Coronavirus Infections - diagnosis
/ Coronavirus Infections - epidemiology
/ COVID-19
/ Enzyme-Linked Immunosorbent Assay - methods
/ Genomes
/ Humans
/ Immunoenzyme Techniques - methods
/ Kinases
/ Nucleic Acid Amplification Techniques - methods
/ Nucleic Acid Amplification Techniques - standards
/ Patients
/ Pneumonia, Viral - diagnosis
/ Pneumonia, Viral - epidemiology
/ Protein Array Analysis - methods
/ Proteins
/ Reverse Transcriptase Polymerase Chain Reaction - methods
/ Review
/ RT-PCR
/ Severe acute respiratory syndrome coronavirus 2
/ Symptom Assessment - methods
/ Viruses
