Journal Article

Green Synthesis of Silver Nanoparticle from Anadenanthera colubrina Extract and Its Antimicrobial Action against ESKAPEE Group Bacteria

Germiniani-Cardozo, Jennifer, Andrade, Gabriel Jonathan Sousa, Souza, Lucas Marcelino dos Santos, Deonas, Anastácia Nikolaos, Nakazato, Gerson, Dahmer, Débora, de Oliveira, Admilton Gonçalves, Torezan, José Marcelo Domingues, Kobayashi, Renata Katsuko Takayama

2024

Overview

Given the urgent need for novel methods to control the spread of multidrug-resistant microorganisms, this study presents a green synthesis approach to produce silver nanoparticles (AgNPs) using the bark extract from Anadenanthera colubrina (Vell.) Brenan var. colubrina. The methodology included obtaining the extract and characterizing the AgNPs, which revealed antimicrobial activity against MDR bacteria. A. colubrina species is valued in indigenous and traditional medicine for its medicinal properties. Herein, it was employed to synthesize AgNPs with effective antibacterial activity (MIC = 19.53–78.12 μM) against clinical isolates from the ESKAPEE group, known for causing high hospitalization costs and mortality rates. Despite its complexity, AgNP synthesis is an affordable method with minimal environmental impacts and risks. Plant-synthesized AgNPs possess unique characteristics that affect their biological activity and cytotoxicity. In this work, A. colubrina bark extract resulted in the synthesis of nanoparticles measuring 75.62 nm in diameter, with a polydispersity index of 0.17 and an average zeta potential of −29 mV, as well as low toxicity for human erythrocytes, with a CC50 value in the range of 961 μM. This synthesis underscores its innovative potential owing to its low toxicity, suggesting applicability across several areas and paving the way for future research.

• Publisher: MDPI AG,MDPI
• Subject: Anadenanthera colubrina / Antibacterial activity / Antibiotics / Antiinfectives and antibacterials / Antimicrobial activity / Antimicrobial agents / Bacteria / Bacterial infections / Bark / Beans / biogenic Bio-AgNPs / Biological activity / Biological effects / Care and treatment / Chemical properties / Clinical isolates / Control methods / Cytotoxicity / Drug resistance / E coli / Environmental impact / Erythrocytes / Flavonoids / Fourier transforms / Green chemistry / Health aspects / Infections / Legumes / Materia medica, Vegetable / Medicinal plants / Methods / Microorganisms / Mimosaceae / Multidrug resistance / Multidrug resistant organisms / multidrug-resistant bacteria / Nanoparticles / Nanotechnology / Pathogens / Plant extracts / Polydispersity / R&D / Research & development / Silver / Spectrum analysis / Synthesis / Toxicity / white angico / Zeta potential