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Engineering an efficient and bright split Corynactis californica green fluorescent protein
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Engineering an efficient and bright split Corynactis californica green fluorescent protein
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Journal Article
Engineering an efficient and bright split Corynactis californica green fluorescent protein
2021
Overview
Split green fluorescent protein (GFP) has been used in a panoply of cellular biology applications to study protein translocation, monitor protein solubility and aggregation, detect protein–protein interactions, enhance protein crystallization, and even map neuron contacts. Recent work shows the utility of split fluorescent proteins for large scale labeling of proteins in cells using CRISPR, but sets of efficient split fluorescent proteins that do not cross-react are needed for multiplexing experiments. We present a new monomeric split green fluorescent protein (ccGFP) engineered from a tetrameric GFP found in
Corynactis californica,
a bright red colonial anthozoan similar to sea anemones and scleractinian stony corals. Split ccGFP from
C. californica
complements up to threefold faster compared to the original
Aequorea victoria
split GFP and enable multiplexed labeling with existing
A. victoria
split YFP and CFP.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
