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An end-to-end pipeline for succinic acid production at an industrially relevant scale using Issatchenkia orientalis
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An end-to-end pipeline for succinic acid production at an industrially relevant scale using Issatchenkia orientalis
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Journal Article
An end-to-end pipeline for succinic acid production at an industrially relevant scale using Issatchenkia orientalis
2023
Overview
Microbial production of succinic acid (SA) at an industrially relevant scale has been hindered by high downstream processing costs arising from neutral pH fermentation for over three decades. Here, we metabolically engineer the acid-tolerant yeast
Issatchenkia orientalis
for SA production, attaining the highest titers in sugar-based media at low pH (pH 3) in fed-batch fermentations, i.e. 109.5 g/L in minimal medium and 104.6 g/L in sugarcane juice medium. We further perform batch fermentation using sugarcane juice medium in a pilot-scale fermenter (300×) and achieve 63.1 g/L of SA, which can be directly crystallized with a yield of 64.0%. Finally, we simulate an end-to-end low-pH SA production pipeline, and techno-economic analysis and life cycle assessment indicate our process is financially viable and can reduce greenhouse gas emissions by 34–90% relative to fossil-based production processes. We expect
I. orientalis
can serve as a general industrial platform for production of organic acids.
Microbial production of succinic acid at an industrially relevant scale has been hindered by high downstream processing costs arising from neutral pH fermentation. Here, the authors report an end-to-end pipeline for succinic acid production at low pH using engineered acid-tolerant
Issatchenkia orientalis
strain.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
