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Rapid empirical discovery of optimal peptides for targeted proteomics
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Journal Article
Rapid empirical discovery of optimal peptides for targeted proteomics
2011
Overview
An empirical approach for identifying optimal proteotypic peptides and fragmentation patterns from
in vitro
–synthesized proteins, for targeted proteomics applications, is described.
We report a method for high-throughput, cost-efficient empirical discovery of optimal proteotypic peptides and fragment ions for targeted proteomics applications using
in vitro
–synthesized proteins. We demonstrate the approach using human transcription factors, which are typically difficult, low-abundance targets and empirically derived proteotypic peptides for 98% of the target proteins. We show that targeted proteomic assays developed using our approach facilitate robust
in vivo
quantification of human transcription factors.
Publisher
Nature Publishing Group US,Nature Publishing Group
