Journal Article

Methanol induces cytosolic calcium variations, membrane depolarization and ethylene production in arabidopsis and tobacco

Tomonori Kawano, Delphine Arbelet-Bonnin, Takashi Kadono, Arnaud Lehner, Tingting Zhao, Aurélien Dauphin, Rafik Errakhi, François Bouteau, Patrice Meimoun, Hieu T H Nguyen, Daniel Tran

2018

Overview

Abstract Background and Aims Methanol is a volatile organic compound released from plants through the action of pectin methylesterases (PMEs), which demethylesterify cell wall pectins. Plant PMEs play a role in developmental processes but also in responses to herbivory and infection by fungal or bacterial pathogens. However, molecular mechanisms that explain how methanol could affect plant defences remain poorly understood. Methods Using cultured cells and seedlings from Arabidopsis thaliana and tobacco BY2 expressing the apoaequorin gene, allowing quantification of cytosolic Ca2+, a reactive oxygen species (ROS) probe (CLA, Cypridina luciferin analogue) and electrophysiological techniques, we followed early plant cell responses to exogenously supplied methanol applied as a liquid or as volatile. Key Results Methanol induces cytosolic Ca2+ variations that involve Ca2+ influx through the plasma membrane and Ca2+ release from internal stores. Our data further suggest that these Ca2+ variations could interact with different ROS and support a signalling pathway leading to well known plant responses to pathogens such as plasma membrane depolarization through anion channel regulation and ethylene synthesis. Conclusions Methanol is not only a by-product of PME activities, and our data suggest that [Ca2+]cyt variations could participate in signalling processes induced by methanol upstream of plant defence responses.

• Publisher: Oxford University Press (OUP),Oxford University Press
• Subject: [CHIM.POLY]Chemical Sciences/Polymers / [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry / [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] / [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry / [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology / [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry / [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] / [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry / [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN] / [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] / [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] / [SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis / [SDV.BIO]Life Sciences [q-bio]/Biotechnology / [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding / [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy / Aequorin / Aequorin - metabolism / Apoproteins / Apoproteins - metabolism / Arabidopsis / Arabidopsis - drug effects / Arabidopsis - physiology / Arabidopsis thaliana / Biochemistry / Biochemistry, Molecular Biology / Biotechnology / calcium / Calcium - metabolism / Cell Behavior / Cell Membrane / Cell Membrane - physiology / cell walls / Cells, Cultured / Cellular Biology / Chemical Sciences / cultured cells / Cytosol / Cytosol - metabolism / Development Biology / electrophysiology / ethylene / ethylene production / Ethylenes / Ethylenes - metabolism / fungi / Gametogenesis / genes / Genomics / herbivores / Life Sciences / luciferin / methanol / Methanol - administration & dosage / Molecular biology / Molecular Biology/Biochemistry [q-bio.BM] / Molecular Biology/Genomics [q-bio.GN] / Molecular Biology/Molecular biology / Molecular Biology/Molecular Networks [q-bio.MN] / Molecular Networks / Nicotiana / Nicotiana - drug effects / Nicotiana - physiology / Nicotiana tabacum / Original / pathogens / pectinesterase / pectins / Phytopathology and phytopharmacy / Plant breeding / Plant Growth Regulators / Plant Growth Regulators - metabolism / Plant Proteins / Plant Proteins - metabolism / plant response / plasma membrane / Polymers / Reactive Oxygen Species / Reactive Oxygen Species - metabolism / Recombinant Proteins / Recombinant Proteins - metabolism / Seedlings / Seedlings - drug effects / Seedlings - physiology / signal transduction / signalization / Subcellular Processes / tobacco / Vegetal Biology / volatile / volatile organic compounds