Asset Details
Do you wish to reserve the book?
Analysis of enteric nervous system and intestinal epithelial barrier to predict complications in Hirschsprung’s disease
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Analysis of enteric nervous system and intestinal epithelial barrier to predict complications in Hirschsprung’s disease
Do you wish to request the book?
Analysis of enteric nervous system and intestinal epithelial barrier to predict complications in Hirschsprung’s disease
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Analysis of enteric nervous system and intestinal epithelial barrier to predict complications in Hirschsprung’s disease
2020
Overview
In Hirschsprung’s disease (HSCR), postoperative course remains unpredictable. Our aim was to define predictive factors of the main postoperative complications: obstructive symptoms (OS) and Hirschsprung-associated enterocolitis (HAEC). In this prospective multicentre cohort study, samples of resected bowel were collected at time of surgery in 18 neonates with short-segment HSCR in tertiary care hospitals. OS and HAEC were noted during postoperative follow-up. We assessed the enteric nervous system and the intestinal epithelial barrier (IEB) in ganglionic segments by combining immunohistochemical, proteomic and transcriptomic approaches, with functional ex vivo analysis of motility and para/transcellular permeability. Ten HSCR patients presented postoperative complications (median follow-up 23.5 months): 6 OS, 4 HAEC (2 with OS), 2 diarrhoea (without OS/HAEC). Immunohistochemical analysis showed a significant 41% and 60% decrease in median number of nNOS-IR myenteric neurons per ganglion in HSCR with OS as compared to HSCR with HAEC/diarrhoea (without OS) and HSCR without complications (
p
= 0.0095;
p
= 0.002, respectively). Paracellular and transcellular permeability was significantly increased in HSCR with HAEC as compared to HSCR with OS/diarrhoea without HAEC (
p
= 0.016;
p
= 0.009) and HSCR without complications (
p
= 0.029;
p
= 0.017). This pilot study supports the hypothesis that modulating neuronal phenotype and enhancing IEB permeability may treat or prevent postoperative complications in HSCR.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 692/53
/ Diarrhea
/ Diarrhea - prevention & control
/ Enteric Nervous System - physiopathology
/ Enterocolitis - epidemiology
/ Enterocolitis - prevention & control
/ Hirschsprung Disease - surgery
/ Humanities and Social Sciences
/ Humans
/ Hépatology and Gastroenterology
/ Infant
/ Intestinal Mucosa - innervation
/ Intestinal Mucosa - physiopathology
/ Neonates
/ Postoperative Complications - epidemiology
/ Postoperative Complications - etiology
/ Postoperative Complications - prevention & control
/ Science
/ Surgery
