Journal Article

Apoplastic class III peroxidases PRX62 and PRX69 promote Arabidopsis root hair growth at low temperature

Fusari, Corina M., Borassi, Cecilia, Servi, Lucas, Ferrero, Lucía, Kasulin, Luciana, Aptekmann, Ariel. A., Rodríguez-Garcia, Diana Rosa, Peralta, Juan Manuel, Petrillo, Ezequiel, Estevez, José M., Gabarain, Victoria Berdion, Meneses, Claudio, Ariel, Federico, Botto, Javier F., Dunand, Christophe, Pacheco, Javier Martínez, Ranocha, Philippe, Marzol, Eliana, Sardoy, Mariana Carignani, Nadra, Alejandro D., del Carmen Rondón Guerrero, Yossmayer

2022

Overview

Root Hairs (RHs) growth is influenced by endogenous and by external environmental signals that coordinately regulate its final cell size. We have recently determined that RH growth was unexpectedly boosted when Arabidopsis thaliana seedlings are cultivated at low temperatures. It was proposed that RH growth plasticity in response to low temperature was linked to a reduced nutrient availability in the media. Here, we explore the molecular basis of this RH growth response by using a Genome Wide Association Study (GWAS) approach using Arabidopsis thaliana natural accessions. We identify the poorly characterized PEROXIDASE 62 (PRX62) and a related protein PRX69 as key proteins under moderate low temperature stress. Strikingly, a cell wall protein extensin (EXT) reporter reveals the effect of peroxidase activity on EXT cell wall association at 10 °C in the RH apical zone. Collectively, our results indicate that PRX62, and to a lesser extent PRX69, are key apoplastic PRXs that modulate ROS-homeostasis and cell wall EXT-insolubilization linked to RH elongation at low temperature. Arabidopsis root hair growth is enhanced at low temperatures. Here the authors show that the class III peroxidases PRX62 and PRX69 modulate ROS homeostasis and cell wall characteristics, and promote root hair elongation at low temperature.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 14/19 / 45/43 / 45/91 / 631/449/1659 / 631/449/2661/2665 / 82/80 / 96/35 / Arabidopsis / Arabidopsis - metabolism / Arabidopsis Proteins - genetics / Arabidopsis Proteins - metabolism / Arabidopsis thaliana / Cell size / Cell walls / Elongation / Extensin / Genome-wide association studies / Genome-Wide Association Study / Genomes / Homeostasis / Humanities and Social Sciences / Life Sciences / Low temperature / multidisciplinary / Nutrient availability / Peroxidase / Peroxidases - genetics / Peroxidases - metabolism / Plant Roots - metabolism / Proteins / Root hairs / Science / Science (multidisciplinary) / Seedlings / Temperature / Vegetal Biology