Asset Details
Do you wish to reserve the book?
Efficient genome editing in dicot plants using calreticulin promoter-driven CRISPR/Cas system
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Efficient genome editing in dicot plants using calreticulin promoter-driven CRISPR/Cas system
Do you wish to request the book?
Efficient genome editing in dicot plants using calreticulin promoter-driven CRISPR/Cas system
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Efficient genome editing in dicot plants using calreticulin promoter-driven CRISPR/Cas system
2025
Overview
K Gene structure and target sites of lettuce LsPDS gene. Since the deletion of chromosomal segments in plants plays a crucial role in the creation of novel plant germplasm (Duan et al. 2021), we developed pDC45 dual sgRNA (pDC45_dsg) systems to achieve multiplex genome editing in tobacco. Among 23 T0 plants, 65.2% and 34.8% of lines contained ho/bi mutations at FAD2-2 and CLC sites, respectively, indicating that pDC45_dsg system improved editing activities at sites with poor sgRNA efficiency (Table S2). [...]we examined dual gRNAs simultaneously targeted to NtBRC1 and NtCCD8 genes, which are correlated to outgrowth of axillary buds in tobacco (Fig. To confirm our hypothesis, we evaluated the editing efficiency of the pDC45 expression system in lettuce (Lactuca sativa), a globally cultivated vegetable crop and another member of the dicotyledonous plants.
