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Changes in Cytoplasmic Volume Are Sufficient to Drive Spindle Scaling

by Gerow, Ken , Hazel, James , Tomschik, Miroslav , Gatlin, J. C. , Oakey, John , Krutkramelis, Kaspars , Mooney, Paul

in Animals / Availability / Biophysics / Cell Division / Cell Extracts / Cell Nucleus / Cell separation / Cell Size / Cells / Circles / Compartments / Cytoplasm / Cytoplasm - chemistry / Cytoplasm - physiology / Developmental stages / Diameters / Eggs / Embryonic Development / Encapsulation / Female / Geometric shapes / Hazel / In vivo tests / Male / Microfluidics / Microfluidics - methods / mitosis / Mitotic spindle apparatus / Movies / Organelles / Ovum - chemistry / Slugs / Spheres / Spindle Apparatus - chemistry / Spindle Apparatus - physiology / Spindle Apparatus - ultrastructure / Spindles / tubulin / Xenopus

2013

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Changes in Cytoplasmic Volume Are Sufficient to Drive Spindle Scaling

by Gerow, Ken , Hazel, James , Tomschik, Miroslav , Gatlin, J. C. , Oakey, John , Krutkramelis, Kaspars , Mooney, Paul

2013

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Changes in Cytoplasmic Volume Are Sufficient to Drive Spindle Scaling

by Gerow, Ken , Hazel, James , Tomschik, Miroslav , Gatlin, J. C. , Oakey, John , Krutkramelis, Kaspars , Mooney, Paul

2013

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Journal Article

Changes in Cytoplasmic Volume Are Sufficient to Drive Spindle Scaling

Gerow, Ken,

Hazel, James,

Tomschik, Miroslav,

Gatlin, J. C.,

Oakey, John,

Krutkramelis, Kaspars,

Mooney, Paul

2013

Overview

The mitotic spindle must function in cell types that vary greatly in size, and its dimensions scale with the rapid, reductive cell divisions that accompany early stages of development. The mechanism responsible for this scaling is unclear, because uncoupling cell size from a developmental or cellular context has proven experimentally challenging. We combined microfluidic technology with Xenopus egg extracts to characterize spindle assembly within discrete, geometrically defined volumes of cytoplasm. Reductions in cytoplasmic volume, rather than developmental cues or changes in cell shape, were sufficient to recapitulate spindle scaling observed in Xenopus embryos. Thus, mechanisms extrinsic to the spindle, specifically a limiting pool of cytoplasmic component(s), play a major role in determining spindle size.

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American Association for the Advancement of Science,The American Association for the Advancement of Science

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